We narrowed to 85,295 results for: Mal;

1. pLenti6.3-efSaCas9-BlastR

   Plasmid

   #220971

   Purpose

   Expresses human codon-optimized efSaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   efSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK-flag-USP19 1-1290

   Plasmid

   #78579

   Purpose

   Expression flag-USP19 1-1290 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 1-1290 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 1291 residue to stop condon

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pXPR_212

   Plasmid

   #133457

   Purpose

   U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ef1a_NANOG_Ires_Puro

   Plasmid

   #18920

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   NANOG (NANOG Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCI-neo-μ1A-(HA)3

   Plasmid

   #198177

   Purpose

   Expression of HA-tagged AP-1 μ1A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1A
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sa-SlugCas9

   Plasmid

   #163797

   Purpose

   Expresses Sa-SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-S9

   Plasmid

   #132948

   Purpose

   Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S9 (rpsI E.coli)
   

   Use

   Tags

   N-terminal 6xHis-tag

   Expression

   Bacterial

   Mutation

   E52C

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMET7-GAG-GW

    Plasmid

    #194634

    Purpose

    Gateway destination vector for mammalian expression of Virotrap bait constructs

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    PQS1

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

    Plasmid

    #199347

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-3C

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pMET7-GAG-HRAS

    Plasmid

    #80604

    Purpose

    Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    HIV-1 p55 GAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGH156_MS2-AID-Hygro

    Plasmid

    #85415

    Purpose

    lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. [Bla][IRES][GFP]

    Plasmid

    #97193

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-mPer2ER-pBABEpuro

    Plasmid

    #61430

    Purpose

    Express mPER2-ER* fusion protein

    Depositor

    Aziz Sancar

    Article

    Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

    Insert

    mPER2 (Per2 Mouse)
    

    Use

    Retroviral

    Tags

    Flag and mouse estrogen receptor ligand binding d…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1 (+) Tacδ

    Plasmid

    #81177

    Purpose

    Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    Tacδ
    

    Use

    Tags

    KLGFFKR

    Expression

    Mammalian

    Mutation

    CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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