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Ddo_innexin-10

Plasmid

#107633

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

innexin-10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pXP220

Plasmid

#26832

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pXP420

Plasmid

#26844

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pBLO 9.3_bCas9-CP230

Plasmid

#121497

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMYT013

Plasmid

#180666

Purpose

Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet5

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->sGFP_PGK-->puroR

Plasmid

#162156

Purpose

Lentiviral vector for constitutive expression of GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMevB

Plasmid

#17819

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pHR_TRE-->2xNLS_IFP_PGK_PuroR

Plasmid

#162238

Purpose

Lentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

NLS IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40

Plasmid

#100030

Purpose

AAV expression of ArcLightD

Depositor

Lawrence Cohen

Article

Storace et al Sci Rep. 2015 May 13;5:10212. doi: 10.10

Insert

ArcLightD

Use

AAV

Tags

2A-mCherry

Expression

Mammalian

Promoter

CBA

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pXP318

Plasmid

#26837

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600uDelta13V

Plasmid

#18014

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) VSFP2.1

Plasmid

#16255

Depositor

Thomas Knopfel

Article

Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Insert

VSFP2.1

Expression

Mammalian

Available Since

Jan. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pXP118

Plasmid

#26825

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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Pgr_opsin

Plasmid

#107655

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

opsin

Use

RNAi; In situ hybridization

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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fSAP24-GST

Plasmid

#11202

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

SAP24 (THOC7 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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p367_DG

Plasmid

#234689

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tag

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

2X Strep tag and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p360_DG

Plasmid

#234703

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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