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Plasmid#134922PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.DepositorInsertCascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA
Plasmid#134924PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo
Plasmid#170321PurposeExpresses HA and mCherry tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA
Plasmid#134923PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.DepositorInsertCascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro
Plasmid#170318PurposeExpresses FLAG and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor
Plasmid#193469PurposeCp36 Donor with promoter for mcherry and puro expressionDepositorInsertCp36_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo
Plasmid#170319PurposeExpresses HA and EGFP tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
Plasmid#176630PurposeIntegrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell linesDepositorInsertEF1a-TET3G-P2A-ERT2-Gal4-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP
Plasmid#118594PurposeParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertsgRNA/Puro-T2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AlphaAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA
Plasmid#20296PurposeCre-activated AAV expression of EYFPDepositorInsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2009AvailabilityAcademic Institutions and Nonprofits only