We narrowed to 13,587 results for: TIM

1. pCMV dCas9-MC (eGFP)

   Plasmid

   #89931

   Purpose

   Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deactivated Cas9, fragment of M.SssI (residues 27…

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SPHK2 gRNA (BRDN0001146242)

   Plasmid

   #77095

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PLK1 gRNA (BRDN0001145759)

   Plasmid

   #76457

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-C/EBPbZIP (pGEX-bZIPa)

   Plasmid

   #12435

   Depositor

   Dong-Er Zhang

   Article

   Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

   Insert

   68 a.a. fragment of C/EBP alpha (CEBPA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5FRTTO-NHASt-codoptUS2

   Plasmid

   #135102

   Purpose

   Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 US2 codon optimized
   

   Use

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-sbGLuc-CheRiff-EGFP

   Plasmid

   #114108

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

   Insert

   Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ADCK4 gRNA (BRDN0001145019)

   Plasmid

   #76560

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ADCK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ADCK4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PLK1 gRNA (BRDN0001144995)

   Plasmid

   #76459

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMuLE ENTR SV40-hCas9 L3-L2

   Plasmid

   #62133

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   human codon optimized Cas9
   

   Use

   CRISPR; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCNL-C1_fibrillarin

    Plasmid

    #65709

    Purpose

    Expresses CNL-fibrillarin in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    fibrillarin (FBL Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLNCX2-mCherry-CHMP2A-siRNAres

    Plasmid

    #115332

    Purpose

    expresses siRNA-resistant mCherry-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mutated to be resistant to siRNA knockdown

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PLK1 gRNA (BRDN0001144743)

    Plasmid

    #76458

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PTK6 gRNA (BRDN0001149364)

    Plasmid

    #76490

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK6

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK6 (PTK6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-BAP-Sox2

    Plasmid

    #133281

    Purpose

    Expression of target proteins BAP-Sox2 in mammalian cells

    Depositor

    Arman Kulyyassov

    Article

    Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Tags

    Biotin Acceptor Peptide contains 7-His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PIP5K1A gRNA (BRDN0001147757)

    Plasmid

    #77963

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PIP5K1A gRNA (BRDN0001147420)

    Plasmid

    #77964

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PIP5K1B gRNA (BRDN0001147624)

    Plasmid

    #77083

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1B (PIP5K1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PLK1 gRNA (BRDN0001147678)

    Plasmid

    #76460

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. INCTbiosyn-pAct-AtINT4

    Plasmid

    #127530

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase 4.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 4 coding sequence codon optimized for A. thaliana expression.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sTR056

    Plasmid

    #177369

    Purpose

    Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

    Depositor

    Jean-Loup Faulon, Jerome Bonnet

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jean-Loup Faulon, Jerome Bonnet

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-45b-NFYC-IDR-mEGFP

    Plasmid

    #185015

    Purpose

    Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    NFYC IDR (Nfyc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NUAK1 gRNA (BRDN0001145557)

    Plasmid

    #75575

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-HA-FbxO10-pcDNA3.1-

    Plasmid

    #52508

    Purpose

    expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO10 (FBXO10 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ROCK2 gRNA (BRDN0001146481)

    Plasmid

    #76350

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. T7T3-PacI-elfn1_459to1199

    Plasmid

    #87671

    Purpose

    In situ probe for mouse Elfn1

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Elfn1 (Elfn1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial sequence, (459 to 1199bp off NM_175522.3)

    Promoter

    T7/T3

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PEAK1 gRNA (BRDN0001145890)

    Plasmid

    #75853

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PEAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PEAK1 (PEAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. non-targeting control gRNA (BRDN0001145269)

    Plasmid

    #80249

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge