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Plasmid#120569PurposeExpresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5 EDD C2768A
Plasmid#37192DepositorInsertEDD C2768A (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationChanged Cysteine 2768 to Alanine; K503R (please s…PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe H-Ras 12V
Plasmid#12545DepositorInsertH-Ras 12V (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V constituitively activated.PromoterAvailable sinceSept. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3
Plasmid#170175PurposeCation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2f-ST-P2A-NLS-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q
Plasmid#84349PurposeExpression of the human Huntingtin Exon1 protein containing 23Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21
Plasmid#78783PurposeRetroviral expression of p21DepositorInsertp21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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PARD3_PDZ_3
Plasmid#103959PurposeProtein expression and purification of PAR3 PDZ3 domainDepositorInsertPARD3 (PARD3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-2
Plasmid#72344PurposeMammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF3-3xHA-pGK-Pur
Plasmid#69053Purposelentiviral expression of IKZF3 with HA tagDepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUBCAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-QES.i03.c01-TM
Plasmid#111845PurposeMammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 Env (BG505 SOSIP.664)
UseTagsCD5 leader sequence and TM helix from MHC class IExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP
Plasmid#198352PurposePart of Firefly reporter system, encodes Renilla luciferaseDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP160ExpressionMutationD10A;H840A nuclease-deficientPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras 61L
Plasmid#13485DepositorInsertHRas 61L (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation61LPromoterAvailable sinceDec. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3
Plasmid#170161PurposeCation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME-P2A-H2B-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-7Q
Plasmid#84347PurposeExpression of the human Huntingtin Exon1 protein containing 7Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal His6-tagged intein SspExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10333
Plasmid#183955PurposeCAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertNuclease-dead Lb Cas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832APromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1
Plasmid#218524PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2
Plasmid#218525PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fuw-AcrIIA4-P2A-GFP
Plasmid#108247PurposeLentiviral vector to express AcrIIA4-P2A-GFPDepositorInsertAcrIIA4-NLS-HA (NEWENTRY Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUbcAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(G137I)
Plasmid#133274PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalianMutationG137IPromoterCMVd2Available sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 K137R
Plasmid#118353PurposeThis plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.DepositorInserthGATA-1 (GATA1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK137RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CHKA
Plasmid#218540Purposehuman CHKA cDNADepositorInsertCHKA (CHKA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK668
Plasmid#192637PurposeExpresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dCas9-NG ha…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pINTO-NFH::mEzh2
Plasmid#65925PurposeExpression of mouse Ezh2 fused to Flag-HA tag (N-terminus)DepositorInsertEnhancer of zeste homolog 2 (Ezh2 Mouse)
UseTagsFLAG-HAExpressionMammalianMutationPromoterCMV/TO (T-REx) and CMV/TetO2 (T-REx)Available sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129E
Plasmid#36050DepositorInsertalpha-synuclein S129E (SNCA Human)
UseTagsExpressionBacterialMutationS129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD55-T2A-GFP
Plasmid#205450Purposelentiviral plasmid for expression of mouse CD55DepositorInsertmCD55 (Cd55 Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
UseTags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129A
Plasmid#36048DepositorInsertalpha-synuclein S129A (SNCA Human)
UseTagsExpressionBacterialMutationS129APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FPP
Plasmid#203758PurposeEncodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertHRas c-term (HRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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