We narrowed to 11,805 results for: NSI;
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Plasmid#91278PurposeProtein expression and purification of human SH3 domain construct SNX33-1/1DepositorInsertSNX33-1/1 (SNX33 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miRE_shBRD9_508-SV40-GFP
Plasmid#75138PurposeRetroviral expression plasmid encoding shBRD9_508 (targeting human BRD)DepositorInsertshBRD9_508
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG_withTEVsite
Plasmid#124940PurposeExpression of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHisExpressionBacterialMutationwith TEV site between RGG domainsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0109
Plasmid#177042PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfKitlgb
Plasmid#168200PurposeExpress zebrafish Kitlgb, mRNA synthesisDepositorInsertKitlgb (kitlgb Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…ExpressionMammalianMutationI85V, V222I, and D233N- please see depositor comm…Available SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xEGFP-mMeikin (E156R, R159E)
Plasmid#174710PurposeMammalian expression of mouse Meikin with Separase-resistant mutationsDepositorAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOpLexA::mini35s (GB0733)
Plasmid#68180PurposeProvides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid partDepositorInsert4xOpLexA:mini35S
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/SPTBN1-FLT3
Plasmid#168115PurposeMammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemiaDepositorInsertSPTBN1-FLT3
ExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgSLC19A1
Plasmid#102314Purposegenetic depletion of SLC19A1DepositorAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB1omega2_SF (GB0466)
Plasmid#68243PurposeTwister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SF (GB0107)
Plasmid#68233PurposeTwister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ldhA
Plasmid#102288PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.DepositorInsertldhA gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-2/3
Plasmid#91420PurposeProtein expression and purification of human SH3 domain construct NCK1-2/3DepositorInsertNCK1-2/3 (NCK1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHis and mCherryExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-N
Plasmid#170153PurposeAAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertNES-MCP-RESCUE-DDN
UseAAVExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rpsL
Plasmid#89953PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.DepositorInsertrpsL gRNA
ExpressionBacterialPromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorInsertEGFP
TagsHuman EMD transmembrane domain and NLSExpressionMammalianPromoterCAGAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)
Plasmid#191177PurposeMammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).DepositorInsertmCherry:GAPDH.s1:EGFP (frameshifted)
ExpressionMammalianMutationEGFP is frameshiftedPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBASH3A-1/1
Plasmid#91478PurposeProtein expression and purification of human SH3 domain construct UBASH3A-1/1DepositorInsertUBASH3A-1/1 (UBASH3A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA)
Plasmid#123150PurposeMercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLE, PrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cdc42 T17N sp64T
Plasmid#16672DepositorAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)
Plasmid#191157PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp longDepositorInsertmTagBFP2:Bdnf.s1:mCherry
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716Available SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOpLacI:mini35ss (GB0534)
Plasmid#68179PurposeProvides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid partDepositorInsert6xOpLacI:mini35S
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR01-FnCpf1
Plasmid#176529PurposeNuclease Cpf1 adapted for Aspergillus speciesDepositorInsertFnCpf1
UseCRISPR; Fungal expressionPromoterTEF1Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_I770A_6xH
Plasmid#185711PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialMutationChanged I770 to APromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)
Plasmid#36879DepositorInsertstTA2
tdT-3Myc
Tags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
p158
Plasmid#62617PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP104-ChromeQ-mCherry
Plasmid#123125PurposeAAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.DepositorInsertChromeQ-mCherry
UseAAVTagsmCherryExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterGFAP104Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-3/3
Plasmid#91476PurposeProtein expression and purification of human SH3 domain construct NCK1-3/3DepositorInsertNCK1-3/3 (NCK1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(FLLFLNL)
Plasmid#40021DepositorInsertsT (ATG)
GFP
beta-globin intron
Neo
Mutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SASH1-1/1
Plasmid#91347PurposeProtein expression and purification of human SH3 domain construct SASH1-1/1DepositorInsertSASH1-1/1 (SASH1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mSTIM1_1-310-CFP
Plasmid#69551PurposeExpresses a STIM1_1-310 fragment mammalian cellsDepositorInsertmSTIM1_1-310
TagsCyan Fluorescent Protein (CFP)ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SLA2-1/1
Plasmid#91450PurposeProtein expression and purification of human SH3 domain construct SLA2-1/1DepositorInsertSLA2-1/1 (SLA2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK2-2/3
Plasmid#91362PurposeProtein expression and purification of human SH3 domain construct NCK2-2/3DepositorInsertNCK2-2/3 (NCK2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralExpressionMammalianPromoterHuman U6 and mouse U6Available SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
hrGFP-BATS
Plasmid#30496DepositorAvailable SinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only