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We narrowed to 8,354 results for: 11

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Showing: 2821 - 2840 of 8354 results

  4. pNESx2-EGFP-C1-PABDx2-Spo20

    Plasmid
    #220082
    Purpose
    PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export
    Depositor
    Gerry Hammond
    Publication
    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spo20 (SPO20 Budding Yeast)
    Tags
    X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…
    Expression
    Mammalian
    Mutation
    amino acids 51-91, tandem dimer
    Promoter
    CMV
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220082-plasmid-map-sequence-id-434081

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  5. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    James Keck
    Publication
    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136472-plasmid-map-sequence-id-267962

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  10. pONSY-CoNMM:mCherry

    Plasmid
    #111878
    Purpose
    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.
    Depositor
    Inaki Ruiz-Trillo
    Publication
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111878-plasmid-map-sequence-id-213490

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  13. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52907
    Purpose
    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Publication
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  18. pODN-mNG-ANLN

    Plasmid
    #183834
    Purpose
    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183834-plasmid-map-sequence-id-362443

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  20. GFP-Tollip M240A,F241A

    Plasmid
    #178051
    Purpose
    GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.
    Depositor
    David Tumbarello
    Publication
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    178051-plasmid-map-sequence-id-350116

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Showing: 2821 - 2840 of 8354 results