We narrowed to 4,936 results for: AAT

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pEPQD0CM0550

Plasmid

#162301

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-SUMO-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0282

Plasmid

#162287

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0024

Plasmid

#162281

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0539

Plasmid

#162306

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, TEV protease (S219V), no stop codon

Use

Synthetic Biology

Mutation

S219V, codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPMY0SP0002

Plasmid

#162304

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, 6xHis tag-[HRV 3C cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0551

Plasmid

#162303

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0547

Plasmid

#162298

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0546

Plasmid

#162297

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, MBP-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0544

Plasmid

#162295

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, MBP-[Factor Xa cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0543

Plasmid

#162294

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, GST-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0541

Plasmid

#162292

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, GST-[thrombin cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-Rictor-shRNA

Plasmid

#157930

Purpose

Knockdown of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor shRNA

Use

Lentiviral and RNAi

Tags

tdTomato

Promoter

mouse U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CANTsg

Plasmid

#154343

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CANT sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAPsg

Plasmid

#154344

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAP sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB1_1

Plasmid

#128242

Purpose

Encodes gRNA for 3' target of human ZBTB1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB1 gRNA

Use

CRISPR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ZHX3_iso1_1

Plasmid

#128249

Purpose

Encodes gRNA for 3' target of human ZHX3_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZHX3_iso1 gRNA

Use

CRISPR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPCR-Script-mLIP

Plasmid

#49194

Purpose

initial cloning vector for mLIP fragment of C/EBPbeta

Depositor

Jed Friedman

Insert

C/EBPbeta (LIP fragment) (Cebpb Mouse)

Use

Cloning vector

Expression

Bacterial

Mutation

only the LIP fragment

Promoter

lac

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pBSU6-shScr/CMV-eGFP

Plasmid

#89912

Purpose

Express shRNA Scrambled control

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Scrambled

Tags

eGFP under separate promoter

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJYS2_crtYf

Plasmid

#85544

Purpose

Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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