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pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

Plasmid

#114085

Purpose

CAG promoter expression plasmid for human codon optimized LbBE1.1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

D832A

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P-Kv

Plasmid

#179462

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPD1411 Simple SV40 HBS

Plasmid

#253894

Purpose

Integration Vector for LumiScarlet with the HBS(SV40_min) promoter (TUPV1), and AkaLuc-P2A-mTagBFP2-P2A-BlastR with the CAG promoter (TUPV2)

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

LumiScarlet, AkaLuc-P2A-mTagBFP-P2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

HBS(SV40_min), CAG

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1413 Simple YBTATAHBS

Plasmid

#253896

Purpose

Integration Vector for LumiScarlet with the HBS(YB_TATA) promoter (TUPV1), and AkaLuc-P2A-mTagBFP2-P2A-BlastR with the CAG promoter (TUPV2)

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

LumiScarlet, AkaLuc-P2A-mTagBFP-P2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

HBS(YB_TATA), CAG

Available Since

April 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1291 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU1

Plasmid

#253918

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1295 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU5

Plasmid

#253922

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1292 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU2

Plasmid

#253919

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1294 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU4

Plasmid

#253921

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1293 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU3

Plasmid

#253920

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1 (ΔExchanger)-PA

Plasmid

#249414

Purpose

Expression vector of mouse Slc9c1(aa588-1175) tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Mutation

deleted amino acids 1-587

Promoter

CAG

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-ARch3.0:EYFP

Plasmid

#248667

Purpose

Expresses Archaerhodopsin3 tagged to eYFP in Cre-expressing cells under the CAG promoter for neuronal inhibition.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

Arch3.0-EYFP

Use

AAV

Tags

eYFP

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouseSlc9c1(ΔExchanger/ΔVSD (S1-S3))-PA

Plasmid

#249413

Purpose

Expression vector of mouse Slc9c1(aa733-1175) tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Mutation

deleted amino acids 1-732

Promoter

CAG

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1 (C-term)-PA

Plasmid

#249415

Purpose

Expression vector of mouse SLC9C1 (aa1026-1175) tagged with PA at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Mutation

deleted amino acids 1-1025

Promoter

CAG

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-human SLC9C1-3xFlag

Plasmid

#249411

Purpose

Expression vector of human SLC9C1 tagged with 3xFLAG at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

SLC9C1 (SLC9C1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Tmem217-3xFlag

Plasmid

#249409

Purpose

Expression vector of mouse Tmem217 tagged with 3xFLAG at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Transmembrane protein 217 (Tmem217 Mouse)

Expression

Mammalian

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1-PA

Plasmid

#249412

Purpose

Expression vector of mouse SLC9C1 tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-HaloTag

Plasmid

#247345

Purpose

Expresses H2B-Halo under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-human GALNTL5-3xHA

Plasmid

#241431

Purpose

The nucleotides coding human GALNTL5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (GALNTL5 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Galntl5 variant #1-3xHA

Plasmid

#240645

Purpose

The nucleotides coding mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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