159,258 results

1. MSI2-ADA

   Plasmid

   #166967

   Purpose

   Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

   Depositor

   Michael Kharas

   Article

   Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

   Insert

   MSI2-ADA
   

   Use

   Retroviral

   Tags

   MSI2-ADA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

   Plasmid

   #221360

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. AAVS1-CAG-hrGFP

   Plasmid

   #52344

   Purpose

   AAVS1 donor vector for constitutive gene expression

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   hrGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_AGIA-AirID

   Plasmid

   #182210

   Purpose

   Express AirID in mammalian cells.

   Depositor

   Shogo Nakano, Sohei Ito, Akira Nozawa, Tatsuya Sawasaki

   Article

   Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

   Insert

   AGIA-AirID
   

   Use

   Tags

   AGIA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC25

   Plasmid

   #177319

   Purpose

   Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

   Depositor

   Rafal Tomecki

   Article

   Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

   Insert

   TtCutA 240-603
   

   Use

   Tags

   6xHis-SUMOTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-HA-hYTHDC1

   Plasmid

   #85167

   Purpose

   Expression of FLAG-HA-tagged human YTHDC1

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Flag and Ha

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-Cepheid1b-ST-WPRE

   Plasmid

   #214967

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

   Depositor

   Peng Zou

   Article

   Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

   Insert

   Cepheid1b-ST
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Floxed-STOP mCherry

   Plasmid

   #122963

   Purpose

   mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_Floxed-STOP mCherry
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMSCVpuro-DEST

    Plasmid

    #119745

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ER-RpHLuorin2

    Plasmid

    #171717

    Purpose

    Luminal ER expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 targeted to ER
    

    Use

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF2053

    Plasmid

    #142912

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-APP-mGFP

    Plasmid

    #196696

    Purpose

    Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    HA and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330-PITCh-LMNB1

    Plasmid

    #207770

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK381 (AAVS1 CMV-OsTIR1F74G)

    Plasmid

    #140536

    Purpose

    AAVS1 CMV-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-UbC-VP64-dCas9-VP64

    Plasmid

    #232179

    Purpose

    Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    VP64-dCas9-VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge