We narrowed to 11,805 results for: NSI;

Showing: 5681 - 5720 of 11805 results

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pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

Plasmid

#83286

Purpose

untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

mTurquoise2-FHA2-AURKB substrate-YPet

Use

Retroviral

Expression

Mammalian

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPAtUbq10 (GB0223)

Plasmid

#68174

Purpose

Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq10 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pXW109ABS84(RS)

Plasmid

#123155

Purpose

Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR-ABS84, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJK526

Plasmid

#155387

Purpose

PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-1/3

Plasmid

#91472

Purpose

Protein expression and purification of human SH3 domain construct NCK1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-1/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Barkor Δ10

Plasmid

#30500

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

1-483aa

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pWP1_mFTCD

Plasmid

#102317

Purpose

expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Ftcd (Ftcd Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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BAS289 C terminal E2C G418R

Plasmid

#74084

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-RUNX2wt-IDR

Plasmid

#145252

Purpose

Expresses fusion of GAL4 DNA-binding domain and RUNX2Awt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2Awt-IDR

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SHANK1-1/1

Plasmid

#91517

Purpose

Protein expression and purification of human SH3 domain construct SHANK1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK1-1/1 (SHANK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-3/3

Plasmid

#91256

Purpose

Protein expression and purification of human SH3 domain construct NCK2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-3/3 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RlACR_477-EYFP

Plasmid

#103771

Purpose

Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_477

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pUWR-Cad-mTFP

Plasmid

#58327

Purpose

Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-mTFP

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
CaM-RhoA(DP)-Venus

Plasmid

#31994

Depositor

Kevin Truong

Article

Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

Insert

CaM-RhoA(DP) (RHOA Human)

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L mutation in RhoA, E7S and D65Y in CaM

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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As1

Plasmid

#123159

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J117, ParsR, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_PTK6-1/1

Plasmid

#91441

Purpose

Protein expression and purification of human SH3 domain construct PTK6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PTK6-1/1 (PTK6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHcPro (GB0067)

Plasmid

#68196

Purpose

Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

TEV HcPro coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-1/4

Plasmid

#91544

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-1/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-2/4

Plasmid

#91457

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-2/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-4/4

Plasmid

#91374

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-4/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-2/2

Plasmid

#91356

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-2/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-3/4

Plasmid

#91366

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-3/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-1/2

Plasmid

#91280

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-1/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-1

Plasmid

#74968

Purpose

sgLacZ-1, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-2

Plasmid

#74969

Purpose

sgLacZ-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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BAS291 C terminal mKO2 kanR

Plasmid

#74086

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLLOXmPOLBeta

Plasmid

#24174

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

pLLOXmPOLbeta (Polb Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

x

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.16

Plasmid

#133536

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3BP4-1/1

Plasmid

#91532

Purpose

Protein expression and purification of human SH3 domain construct SH3BP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3BP4-1/1 (SH3BP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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BAS290 C terminal E2C natR

Plasmid

#74085

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pHH0103_SORBS2-1/3

Plasmid

#91387

Purpose

Protein expression and purification of human SH3 domain construct SORBS2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS2-1/3 (SORBS2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_VAV1-1/2

Plasmid

#91355

Purpose

Protein expression and purification of human SH3 domain construct VAV1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV1-1/2 (VAV1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM549

Plasmid

#112531

Purpose

pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 15, 2020

Availability

Academic Institutions and Nonprofits only

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We narrowed to 11,805 results for: NSI;