We narrowed to 50,495 results for: ang

1. pDONR223-H-RAS V12

   Plasmid

   #31201

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   H-Ras (HRAS Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   G12V constitutively active

   Promoter

   Available Since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

2. 2XSmBiT-SARS-CoV-2 S1

   Plasmid

   #173430

   Purpose

   protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

   Depositor

   David Nemazee

   Article

   Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

   Insert

   2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
   

   Use

   Luciferase

   Tags

   human IgG1 Fc

   Expression

   Mammalian

   Mutation

   furin cleavage site RRAR to GSAS

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF1827

   Plasmid

   #143853

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARNT (ARNT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSICO-CPSF6-4Glu-iRFP670

   Plasmid

   #167588

   Purpose

   Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

   Depositor

   Zandrea Ambrose

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   CPSF6-4Glu-iRFP670 (CPSF6 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   K547E, R549E, R559E, R561E

   Promoter

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTT5-hSDC1-CS1/CS2

   Plasmid

   #52370

   Purpose

   expresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R95Q, S184A and S194A (according to numbering in …

   Promoter

   CMV

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. CL20_mEGFP_CLTC_VMP1

   Plasmid

   #205793

   Purpose

   Express mEGFP-tagged fusion protein, CLTC_VMP1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CLTC_VMP1 (CLTC, VMP1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMThy1.1_hDDX5_FL_D248N

   Plasmid

   #88874

   Purpose

   constitutive expression of DDX5 D248N in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D248N

   Promoter

   MSCV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-Flag-Polß(D256A)-IRES-puro

   Plasmid

   #128677

   Purpose

   mammalian expression of Flag-Polß(D256A) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D256A

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF1825

    Plasmid

    #143322

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNT (ARNT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-Flag-Polß(WT)-IRES-Neo

    Plasmid

    #128663

    Purpose

    mammalian expression of Flag-Polß(WT) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTT5-hSDC1-CS1

    Plasmid

    #52365

    Purpose

    expresses human Syndecan-1 point mutant at S184A in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R95Q and S184A (according to numbering in Fig. 2A…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pTT5-hSDC1-S15A

    Plasmid

    #52363

    Purpose

    expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S15A and R95Q (according to numbering in Fig. 2A …

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

14. TFORF1828

    Plasmid

    #142861

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNT (ARNT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-multi-CRISPR-sgRipk3_#2-puro

    Plasmid

    #231982

    Purpose

    Knockout mouse Ripk3

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-multi-CRISPR-sgRipk3_#1-puro

    Plasmid

    #231981

    Purpose

    Knockout mouse Ripk3

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-multi-CRISPR-sgMlkl_#2-puro

    Plasmid

    #231980

    Purpose

    Knockout mouse Mlkl

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-multi-CRISPR-sgMlkl_#1-puro

    Plasmid

    #231979

    Purpose

    Knockout mouse Mlkl

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX MLKL(1-180)-2A-mCherry-puro

    Plasmid

    #231976

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX MLKL(S345D)-2A-mCherry-puro

    Plasmid

    #231975

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    MLKL S345D

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HNRNPA1-miRFP670-TRF1

    Plasmid

    #227384

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CL20_mEGFP_RPS6KB1_VMP1

    Plasmid

    #205913

    Purpose

    Express mEGFP-tagged fusion protein, RPS6KB1_VMP1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    RPS6KB1_VMP1 (RPS6KB1, VMP1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF1826

    Plasmid

    #143159

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNT (ARNT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-Flag-Polß(V303R)-IRES-puro

    Plasmid

    #128659

    Purpose

    mammalian expression of Flag-Polß(V303R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V303R

    Promoter

    CMV

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. px458-AMBRA1 #3

    Plasmid

    #172607

    Purpose

    Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-Flag-Polß(L301R/V303R)-IRES-neo

    Plasmid

    #128664

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-Flag-Polß(L301R/V303R)-IRES-puro

    Plasmid

    #128658

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-Flag-Polß(K72A)-IRES-puro

    Plasmid

    #128661

    Purpose

    mammalian expression of Flag-Polß(K72A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K72A

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-Flag-Polß(TM)-IRES-hyg

    Plasmid

    #128668

    Purpose

    mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with hygromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-Flag-Polß(K244A)-IRES-puro

    Plasmid

    #128682

    Purpose

    mammalian expression of Flag-Polß(K244A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K244A

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLVX-Flag-Polß(L301R)-IRES-puro

    Plasmid

    #128654

    Purpose

    mammalian expression of Flag-Polß(L301R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLVX-Flag-Polß(K206A)-IRES-puro

    Plasmid

    #128678

    Purpose

    mammalian expression of Flag-Polß(K206A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K206A

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLVX-Flag-Polß(V306R)-IRES-puro

    Plasmid

    #128655

    Purpose

    mammalian expression of Flag-Polß(V306R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V306R

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-Flag-Polß(L301R/V306R)-IRES-puro

    Plasmid

    #128656

    Purpose

    mammalian expression of Flag-Polß(L301R/V306R) with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V306R

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX-Flag-Polß(KTM)-IRES-puro

    Plasmid

    #128672

    Purpose

    mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K41R/K61R/K81R

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVX-Flag-Polß(K206A/K244A)-IRES-puro

    Plasmid

    #128683

    Purpose

    mammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K206A/K244A

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-Flag-Polß(TM)-IRES-neo

    Plasmid

    #128671

    Purpose

    mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MSCV-HA-Nr4a1-Zf2

    Plasmid

    #160944

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Deletion of Zinc finger 2 (C306 to C330)

    Promoter

    MSCV-LTR

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MSCV-HA-Nr4a1-Zf1

    Plasmid

    #160943

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Deletion of Zinc finger 1 (C270 to C290)

    Promoter

    MSCV-LTR

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGEX-2TKN-bp1

    Plasmid

    #133428

    Purpose

    Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    SREBF1 (SREBF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    SREBP1a amino acids 1-50 fused to GST

    Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LSL-Cas9-Rosa26TV

    Plasmid

    #61408

    Purpose

    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    3xFLAG and P2A

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CAG

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCSC-ISL1-T2A-LHX3

    Plasmid

    #90215

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    ISL1-T2A-LHX3 (ISL1, LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCSC-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90214

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pinducer 20 DN-KASHΔPPPL

    Plasmid

    #129280

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

    Depositor

    Daniel Conway

    Article

    Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    the last 4 amino acids (PPPL) of the KASH domain …

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60228

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. PGL4.11-COL1A1WT

    Plasmid

    #230915

    Purpose

    Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    luciferase - Luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge