We narrowed to 59,231 results for: %s

1. pGAL4-DBD-BHLHE41-Adel-IDR

   Plasmid

   #145228

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   BHLHE41-Adel-IDR (BHLHE41 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-Basic-ZC3H12D_PromoterMUT

   Plasmid

   #153067

   Purpose

   ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

   Promoter

   None

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmirGLO-3'UTR-IL22

   Plasmid

   #153070

   Purpose

   Human IL-22 3'UTR region cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   3' UTR region of IL-22 gene (IL22 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX335A_hCas9(D10A)_IRF8gRNA4

   Plasmid

   #89722

   Purpose

   Knockout IRF8 in human cells

   Depositor

   Martin Zenke

   Article

   Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

   Insert

   IRF8 gRNA4 (IRF8 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX335A_hCas9(D10A)_IRF8gRNA3

   Plasmid

   #89721

   Purpose

   Knockout IRF8 in human cells

   Depositor

   Martin Zenke

   Article

   Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

   Insert

   IRF8 gRNA3 (IRF8 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

   Plasmid

   #176285

   Purpose

   Cre-dependent AAV virus

   Depositor

   Shenqin Yao, Allen Institute for Brain Science

   Article

   Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

   Insert

   TVA66T-dTomato-rabies Glycoprotein
   

   Tags

   dTomato

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP5004 pAAV-Syn-DIO-TVA-dTom-SAD-B19G

   Plasmid

   #176286

   Purpose

   Cre-dependent AAV virus

   Depositor

   Shenqin Yao, Allen Institute for Brain Science

   Article

   Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

   Insert

   TVA-dTomato-rabies Glycoprotein
   

   Tags

   dTomato

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP5005 pAAV-Syn-DIO-TVA66T-dTom-SAD-B19G

   Plasmid

   #176287

   Purpose

   Cre-dependent AAV virus

   Depositor

   Shenqin Yao, Allen Institute for Brain Science

   Article

   Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

   Insert

   TVA66T-dTomato-rabies Glycoprotein
   

   Tags

   dTomato

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP5002 RabV-SADdeltaG-histone-EGFP

   Plasmid

   #176284

   Purpose

   Recombinant rabies virus expressing histone-EGFP

   Depositor

   Shenqin Yao, Allen Institute for Brain Science

   Article

   Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

   Insert

   H2B (H2BC11 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CS-TRE-BclXL-Ubc-rtTA

    Plasmid

    #198189

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBCLXL (BCL2L1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid

    #196887

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. hSOD1-FLAG

    Plasmid

    #182922

    Purpose

    Mammalian expression plasmid for human WT SOD1

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    [Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB’

    Plasmid

    #162703

    Purpose

    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Expression

    Bacterial

    Mutation

    5513T>G (tetR E37A); 7758G>T (second tet op…

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-GyrA_F164R238

    Plasmid

    #216230

    Purpose

    S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed arginine 238 to serine, phenylalanine 168…

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET151-GyrB_A467

    Plasmid

    #216234

    Purpose

    S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed alanine 467 to valine

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET151-GyrB_T522

    Plasmid

    #216235

    Purpose

    S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed threonine 522 to isoleucine

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

    Plasmid

    #191527

    Purpose

    Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG) + 3xFLAG_TwinStrep

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. spoT_NS1

    Plasmid

    #100914

    Purpose

    expresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

    Insert

    synpcc7942_1377 (spoT)
    

    Use

    Cyanobacteria cloning vector

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. spoT-

    Plasmid

    #100913

    Purpose

    deletes synpcc7942_1377 (spoT)

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

    Insert

    Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
    

    Use

    Cloning vector

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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