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pLX - T7pro - EBFP

Plasmid

#138615

Purpose

Express EBFP with an upstream T7 promoter in a lentiviral backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EBFP

Use

Lentiviral

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Bamh1

Plasmid

#203303

Purpose

For expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nde1

Plasmid

#203309

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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CIp10 - mTurquoise2 (γmGFP based)

Plasmid

#163121

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

mTurquoise2 - γmGFP based

Use

Candida albicans expression

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-iCRE-2A-H2BGFP-WPRE

Plasmid

#234893

Purpose

CAG-driven Cre recombinase coupled with H2B-GFP for nuclear fluorescent labelling

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

iCRE-2A-H2BGFP

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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ERmoxGFP

Plasmid

#68072

Purpose

mammalian expression of ER localized moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

moxGFP

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pKJ275

Plasmid

#217491

Purpose

RADARSv3 driving luciferase

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

gaussia luciferase

Expression

Mammalian

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAB1620 hU6-BpiI-Cas13bt3-DR

Plasmid

#176315

Purpose

hU6-BpiI-Cas13bt3-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt3 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, NanoLuc-BRD9

Plasmid

#238753

Purpose

Express NanoLuc-BRD9 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

BRD9 (BRD9 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSELIS-RamR

Plasmid

#202631

Purpose

Enables dual growth and fluorescence based enrichment of RamR biosensors

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

Sh ble

Use

Synthetic Biology

Expression

Bacterial

Promoter

Lambda promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ACS1

Plasmid

#13746

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Synthetase 1 (Acss2 Mouse)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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hNedd4-2

Plasmid

#83433

Purpose

full length Nedd4L with His Xpress tag

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

Insert

human Nedd4L with C2 domain (NEDD4L Human)

Tags

His Tag (6x) and Xpress Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-sspB-Sec61

Plasmid

#247297

Purpose

Mammalian expression of mCherry-sspB-Sec61 fusion; for optogenetic control of protein recruitment to the ER

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

mCherry-sspB-Sec61

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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JD638

Plasmid

#160395

Purpose

Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis miR396-resistant GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-NanoLuc

Plasmid

#188247

Purpose

Template plasmid to transcribe mRNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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