We narrowed to 8,979 results for: FIE
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Plasmid#53334PurposeExpresses full-length Nephrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNPHS (NPHS1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
ExpressionMammalianAvailable SinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
TagsGFPExpressionMammalianPromoterCMVAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-hBACCS2
Plasmid#67626PurposeMammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switchDepositorInsertORAI1-hBACCS2
TagsHAExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
Plasmid#172501PurposeEncodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacersDepositorInsertDisCas7-11 full locus with Golden gate acceptor site for spacer
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human NANOS3-P2A-mCherry knock-in targeting construct
Plasmid#59721PurposeGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.DepositorInsertP2A mCherry
ExpressionMammalianAvailable SinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PF4-bio-His
Plasmid#53411PurposeExpresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPF4 (PF4 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PpyCAG-NANOG-IB
Plasmid#60440PurposeTransient human NANOG expressionDepositorInsertNANOG (NANOG Human)
ExpressionMammalianAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-GFP
Plasmid#72891PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and GFPDepositorInserthBACCS2-IRES-GFP
TagsIRES-GFPExpressionMammalianPromoterCMVAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV-Let7d-5p
Plasmid#117055PurposeOverexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expressionDepositorInsertLet-7d-5p - OFP
UseLentiviralPromoterSFFV promoterAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU
Plasmid#31726DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_B6
Plasmid#170300PurposeA knock-out vector for the mouse Ptgs2 in C57BL6DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a
Plasmid#109519PurposeMuscle specific zebrafish expression of mKate2 fused to Dynamin2aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minBG-CI-SaCas9-W3SynpA
Plasmid#231369PurposeminBG-driven SaCas9, with short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minCMVe-SCP1-SaCas9-W3SynpA
Plasmid#231370PurposeSCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tmem38A pEGFP-N2 (1401)
Plasmid#62050Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase
Plasmid#188391PurposeReporter for BCAM expressionDepositorInsertBCAM reporter
UseLuciferaseAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only