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psiCHECK3-EIF3B

Plasmid

#136012

Purpose

EIF3B 3'UTR (NM_001037283.1) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pYSD313

Plasmid

#212931

Purpose

Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

Sed1 (SED1 Budding Yeast)

Expression

Yeast

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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1 kb Axin2 basic

Plasmid

#25701

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
MSP2253

Plasmid

#70704

Purpose

Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP in pDBEcR-pIND(SP1)/Bla

Plasmid

#201557

Purpose

Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc and FLAG-EGFP

Expression

Mammalian

Promoter

EF1a and pIMD(SP1)

Available Since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir-122

Plasmid

#46673

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-122 (MIR122 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

Plasmid

#191397

Purpose

sgRNA targeting alpha-satellites on human chromosome 9

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

sgRNA targeting alpha satellites on human chromosome 9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCIG-Flag-Sox9-IRES-nls-GFP

Plasmid

#44281

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

Flag-Sox9 (SOX9 Chicken)

Tags

Flag

Expression

Mammalian

Promoter

CMV-IE enhancer and Chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5L18-CtermQ7

Plasmid

#51442

Purpose

Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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Flag Snail 4SA

Plasmid

#16220

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
200 bp Axin2 basic

Plasmid

#25703

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-GP-2-DNMT3A PWWP

Plasmid

#59696

Purpose

Contains histone modification interacting domain (DNMT3A PWWP)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

DNMT3A PWWP (Dnmt3a Mouse)

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermQ7

Plasmid

#51443

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-CBX7 Chromo

Plasmid

#59695

Purpose

Contains histone modification interacting domain (CBX7 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX7 Chromo (CBX7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 3SA

Plasmid

#121490

Purpose

Expression of human PD-L1 3SA with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 3SA
humun PD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S176A, T180A, S184A

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-PD-L1 3SA/4NQ

Plasmid

#121491

Purpose

Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMiR-E6AP-3'UTR

Plasmid

#53694

Purpose

Luciferase reporter assay for microRNA binding on E6AP 3'UTR

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

Plasmid

#101205

Purpose

Bacterial expression plasmid for SpCas9 REC3 domain

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K506–Q712

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K506–Q712

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Flag Snail 8SA

Plasmid

#16222

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
cmv hTid Short

Plasmid

#13709

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only