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We narrowed to 5,960 results for: actin

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Showing: 2861 - 2880 of 5960 results

  10. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

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  11. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  12. pAAV.CAG.iAChSnFR-NULL

    Plasmid
    #137956
    Purpose
    Expresses iAChSnFR (non-binding variant) under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding variant)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Y140A binding site mutant
    Promoter
    CAG
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137956-plasmid-map-sequence-id-271715

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  15. gTBEv3

    Plasmid
    #220619
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220619-plasmid-map-sequence-id-436981

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  16. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  18. pHDR-eGFP (pXK-1647)

    Plasmid
    #208824
    Purpose
    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208824-plasmid-map-sequence-id-417244

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  19. AYBEv0.1

    Plasmid
    #193966
    Purpose
    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    Expression
    Mammalian
    Promoter
    hU6, EF1a, CBh
    Available Since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193966-plasmid-map-sequence-id-384658

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  20. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

    Plasmid
    #137960
    Purpose
    Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding yellow variant)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    Y140A binding site mutation
    Promoter
    CAG
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137960-plasmid-map-sequence-id-271575

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Showing: 2861 - 2880 of 5960 results