Addgene

Showing: 2861 - 2880 of 11705 results

Results per page:

Sort results by:

TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Y587E EphA2 pcDNA3

Plasmid

#102730

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y587E (TAT > GAG)

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-N-3XFLAG-Ubac2

Plasmid

#123597

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

ubiquitin associated domain containing 2 (Ubac2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 merlin 1-590

Plasmid

#11624

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2-WT(732)

Plasmid

#154098

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (untagged)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyn-AktAR

Plasmid

#123356

Purpose

FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

Lyn-AktAR

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-SUN1 EGFP

Plasmid

#187675

Purpose

In vivo visualization of SUN1

Depositor

Mary Beckerle

Insert

SUN1 (Sun1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-hTRAF3 333-C

Plasmid

#44035

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Use

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 334-568; S393G and K492N

Promoter

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA-SCW-HHL

Plasmid

#190634

Purpose

Mammalian expression of genetically encoded calcium indicator SCW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW-HHL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Paragranulin-no linker

Plasmid

#176917

Purpose

express Paragranulin without linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_mGFP-FGFR3-G380R

Plasmid

#191755

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Use

Tags

mGFP

Expression

Mammalian

Mutation

c.1138G>A (G380R) + c.1131C>T (silent mutat…

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Kohinoor-Actin/pcDNA3

Plasmid

#67776

Purpose

Actin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 Tat K41A HA (P#37)

Plasmid

#14655

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

K41A

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1: TfR1-CoilE

Plasmid

#181988

Purpose

CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TfR1-CoilE

Use

Tags

CoilE

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-C-6His-mEsrra

Plasmid

#173152

Purpose

mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al bioRxiv 2021.11.16.468891

Insert

ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Paragranulin+linker 1

Plasmid

#176915

Purpose

express Paragranulin with linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin + linker 1 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPE-600n

Plasmid

#13541

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-gLuc-C1C2

Plasmid

#178426

Purpose

Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

gLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge