4,139 results

1. pCMV(MinDis).iGluSnFR

   Plasmid

   #41732

   Purpose

   Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   Myc, PDGFR transmembrane helix (aa 513-561), and …

   Expression

   Mammalian

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   CMV

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-USP15

   Plasmid

   #22570

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP15 (USP15 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLM-mCerulean-cMyc

   Plasmid

   #23244

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Cas9

   Plasmid

   #47322

   Purpose

   For in vivo expression of Cas9 or generation of Cas9 mRNA

   Depositor

   Alex Schier

   Article

   Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

   Insert

   Cas9
   

   Use

   CRISPR; Xenopus and zebrafish expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL-MS2-6X

   Plasmid

   #27118

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (6)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Drp1K38A

   Plasmid

   #45161

   Depositor

   Richard Youle, Alexander van der Bliek

   Article

   Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

   Insert

   Drp1 (DNM1L Human)
   

   Use

   Tags

   HA (AYPYDVPDY)

   Expression

   Mammalian

   Mutation

   K38A

   Promoter

   CMV

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CD68 promoter/enhancer

   Plasmid

   #34837

   Depositor

   Peter Murray

   Article

   Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

   Insert

   CD68 promoter/enhancer -680/+140 (CD68 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-FLAG-LKB1

   Plasmid

   #8592

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-GbL

    Plasmid

    #1865

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    GbL (MLST8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLifeAct_mScarlet-i_N1

    Plasmid

    #85056

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF.PGK.GFP

    Plasmid

    #17618

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-zeo largeTgenomic

    Plasmid

    #1778

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T and small T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA Flag Yap1

    Plasmid

    #18881

    Depositor

    Yosef Shaul

    Article

    Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

    Insert

    Yap1 (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. PSD-95 FLAG

    Plasmid

    #15463

    Depositor

    Wei-dong Yao

    Article

    Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV/SB10

    Plasmid

    #24551

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)/Luc2=tdT

    Plasmid

    #32904

    Purpose

    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

    Depositor

    Christopher Contag

    Article

    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

    Inserts

    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    

    Use

    Tags

    tdTomato red fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE and n/a

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDestTol2pACryGFP

    Plasmid

    #64022

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    GFP
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    alpha-Acrystallin promoter

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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