We narrowed to 26,456 results for: CAL;

1. pTS2386-Tier1-PhCMV-GCaMP6s

   Plasmid

   #169519

   Purpose

   Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM126

   Plasmid

   #105134

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BRAC/pcDNA3

   Plasmid

   #51967

   Purpose

   mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

   Insert

   BRAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

4. PU6::unc-119_sgRNA

   Plasmid

   #46169

   Purpose

   unc-119 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   unc-119 targeting sgRNA (unc-119 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. GreenT-EC - GPI

   Plasmid

   #193946

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   GPI-anchor and Igk_secretion - HA-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-jGCaMP7b-XC

   Plasmid

   #182221

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP7b.

   Depositor

   Xiaodong Liu

   Article

   Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

   Insert

   jGCaMP7b-XC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. nls-Flamindo2

   Plasmid

   #73939

   Purpose

   Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   nls-Flamindo2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. psicheck-2-Acsm2

   Plasmid

   #48158

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of Acsm2 (Acsm2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY2low-mCherry

   Plasmid

   #104065

   Purpose

   Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2low-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2 aa 1-491 truncation, R489E A491D

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKL004

    Plasmid

    #98920

    Purpose

    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    GCaMP6f tethered to mRuby
    

    Use

    Tags

    His and mRuby3

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-miniSOG-C1

    Plasmid

    #54801

    Purpose

    Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. AX53_pS.Donor.S1.TS

    Plasmid

    #100290

    Purpose

    AAVS1-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-INU-FLAG-FAM134Bs

    Plasmid

    #128261

    Purpose

    Expresses FAM134Bs in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134Bs (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-CoilR-Cys

    Plasmid

    #181989

    Purpose

    Used to label proteins with the CoilE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    CoilR-Cys
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-K-I-N-CAAX

    Plasmid

    #41640

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Vinculin_TurboID_Venus

    Plasmid

    #200272

    Purpose

    Expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    Vinculin_TurboID_Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hOC-GFPtpz

    Plasmid

    #110207

    Purpose

    Expresses GFP in mature bone cells.

    Depositor

    David Rowe

    Article

    Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

    Insert

    eGFPtopaz
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Human osteocalcin (BGLAP)

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEm-CoilE-Actin

    Plasmid

    #181993

    Purpose

    CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    mEm-CoilE-Actin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mito-LSSmGFP

    Plasmid

    #186536

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. actc1b- mKate2-KDEL-pA

    Plasmid

    #109494

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b- mKate2-KDEL-pA
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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