We narrowed to 65,871 results for: des.1

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htrpC6-YFP

Plasmid

#21084

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

TrpC6 (TRPC6 Human)

Tags

YFP

Expression

Mammalian

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHEE2E-TRI

Plasmid

#71288

Purpose

Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Inserts

sgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9

Use

CRISPR; Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfralpha-EGFPN

Plasmid

#66787

Purpose

Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP-UtrCH

Plasmid

#26739

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

monomeric RFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG-TREX1

Plasmid

#27218

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-Ikka-HA WT

Plasmid

#23296

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

IKK alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
pLDLR-Luc mutSRE

Plasmid

#14945

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

LDLR promoter mut SRE (LDLR Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Point mutation in the SRE-1 sequence of the LDL r…

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pCMMP-TVA800

Plasmid

#15778

Depositor

Edward Callaway

Article

Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

Insert

TV A800

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Tags

ECFP

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pEX-HcRed-hLC3WT

Plasmid

#24991

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)

Tags

HcRed

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

Plasmid

#118156

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2/pAAV

Plasmid

#91718

Purpose

AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2

Use

AAV

Promoter

CaMKII 1.3

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Synapsin-pHoenix-WPRE

Plasmid

#70111

Purpose

lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)

Use

Lentiviral

Tags

Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

Promoter

Synapsin

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-trCIB1

Plasmid

#64119

Purpose

Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

dCas9-trCIB1 fusion

Tags

HA and NLS

Expression

Mammalian

Mutation

dCas9 (D10A H840A, catalytically inactive), trCIB…

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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GB-NES

Plasmid

#61017

Purpose

ddGFP B-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP B

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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