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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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v5 eVLP gag-pro-pol

Plasmid

#228466

Purpose

Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag-pro-pol(Q226P)

Expression

Mammalian

Mutation

Q226P

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-NLS-HA-TurboID

Plasmid

#215075

Purpose

Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP

Plasmid

#91975

Purpose

EGFP reporter cell line control (no miR-19 sites)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCitrine

Plasmid

#188386

Purpose

Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

Gal4UAS_tBFP_PGK_mCitrine

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b crRNA backbone

Plasmid

#103854

Purpose

For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has Service

Cloning Grade DNA

Insert

U6-BbsI-BbsI-PspCas13b DR-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBBR1k-GFPuv

Plasmid

#106382

Purpose

IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-mStayGold (E138D)

Plasmid

#211363

Purpose

Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Mammalian

Mutation

E138D

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV mCherry-GFP-LC3B WT

Plasmid

#123230

Purpose

Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3A-IRES-Puro

Plasmid

#107349

Purpose

Expresses HA tagged UBE3A in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3A (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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