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pEGFP hsp70 K71E

Plasmid

#15216

Depositor

Lois Greene

Publication

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 K71E (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

K71E, ATPase-deficient mutant.

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CD20

Plasmid

#1890

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

CD20 (MS4A1 Human)

Expression

Mammalian

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Publication

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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903 pLNCX HA Akt1

Plasmid

#9004

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pJR89

Plasmid

#140096

Purpose

sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

Depositor

Jonathan Weissman

Publication

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Publication

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Expression

Mammalian

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-BE1

Plasmid

#73019

Purpose

Expresses BE1 with C-terminal NLS in mammalian cells

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Expression

Mammalian

Promoter

CMV

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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MSCV Puro

Plasmid

#68469

Purpose

Retroviral plasmid with multiple cloning site and puromycin resistance

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Retroviral

Expression

Mammalian

Promoter

pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund, Benjamin Rost

Publication

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Publication

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-PTRE-tight-hM3Dq-mCherry

Plasmid

#66795

Purpose

Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

Depositor

William Wisden

Publication

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

pTIGHT promoter-hM3Dq-mCherry

Use

AAV

Expression

Mammalian

Promoter

pTIGHT TRE

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Publication

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCAG_GPHN.FingR-mKate2-IL2RGTC

Plasmid

#46297

Depositor

Don Arnold

Publication

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Publication

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C (CYCS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Publication

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pFA6a-link-yomRuby2-SpHis5

Plasmid

#44858

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Publication

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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