We narrowed to 3,916 results for: LYC

1. eGFP-KIF1A-Rigor

   Plasmid

   #172207

   Purpose

   Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF1A (Kif1a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 251 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Syn-ATP

   Plasmid

   #51819

   Purpose

   Optical reporter of presynaptic ATP

   Depositor

   Timothy Ryan

   Article

   Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

   Insert

   Chimeric Synaptophysin-mCherry-Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Within WT luciferase gene, changed Threonine 214 …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAaePUb::Cas9-2A-Neo

   Plasmid

   #176680

   Purpose

   Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
   

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Drosophila codon optimized

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTFG005_Spike_Inducible_3xFlag

   Plasmid

   #191578

   Purpose

   Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   SARS-CoV-2 Spike Protein (S Synthetic)
   

   Use

   Lentiviral

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-KRAS4A(G12V)

   Plasmid

   #35634

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   K-Ras4A(G12V) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Glycine 12 to Valine

   Promoter

   PGK

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

6. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. OPEN HLA-A*02:01-BirA

   Plasmid

   #215569

   Purpose

   E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   Glycine 120 to Cysteine

   Promoter

   T7

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV 3xFLAG-LC3B G120A

   Plasmid

   #123093

   Purpose

   Expresses 3xFLAG-LC3B G120A in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHEN-CoV2-nanobody-F1

   Plasmid

   #194592

   Purpose

   Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   F1 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpyTag-RBD

    Plasmid

    #159999

    Purpose

    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHEN-CoV2-nanobody-E2

    Plasmid

    #194590

    Purpose

    Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    E2 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCsy_complex

    Plasmid

    #89232

    Purpose

    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Inserts

    Csy1
    Csy2
    Csy3
    Csy4
    

    Use

    Tags

    6xHis-TEV protease

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AMA1-bio

    Plasmid

    #47741

    Purpose

    Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised AMA1
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

    Plasmid

    #47458

    Purpose

    LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP10-bio

    Plasmid

    #47719

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP10
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV 3xFLAG-GABARAPL2 G116A

    Plasmid

    #123104

    Purpose

    Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HDM_omicron_B11529_IDTDNA

    Plasmid

    #204142

    Purpose

    Expression plasmid for SARS-CoV-2 BA.1 variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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