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Plasmid#218536Purposehuman FLVCR1b with C-terminal 3X_FLAG cDNADepositorInsertFLVCR1 (FLVCR1 Human)
UseRetroviralTagsFLAG (3X)ExpressionMammalianMutationAlternative isoformPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG
Plasmid#218534Purposehuman FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNADepositorInsertFLVCR1 (FLVCR1 Human)
UseRetroviralTagsFLAG (3X)ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+KDM5B-AS1
Plasmid#194203PurposeExpresses lncRNA KDM5B-AS1DepositorInsertKDM5B-AS1 (PCAT6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-poUnaG
Plasmid#220103PurposeFor Agrobacterium-mediated transformation. Peroxisome-localized mCherry-UnaG.DepositorInsertmCherry-UnaG
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-ptUnaG
Plasmid#221660PurposeFor Agrobacterium-mediated transformation. Plastid(stroma)-localized mCherry-UnaG.DepositorInsertmCherry-UnaG
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-mtUnaG
Plasmid#221664PurposeFor Agrobacterium-mediated transformation. Mitochondria-localized mCherry-UnaGDepositorInsertmCherry-UnaG flanked with an N-terminal presequence of F1-ATPase γ-subunit
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-erUnaG
Plasmid#221663PurposeFor Agrobacterium-mediated transformation. Endoplasmic reticulum-localized mCherry-UnaG.DepositorInsertmCherry-UnaG flanked with an N-terminal signal peptide or field pumpkin 25 albumin
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-oeUnaG
Plasmid#221662PurposeFor Agrobacterium-mediated transformation. Plastid(outer envelope membrane)-localized mCherry-UnaG.DepositorInsertmCherry-UnaG flanked with an N-terminal sequence of Arabidopsis OUTRE ENVELOPE PROTEIN7
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-tkUnaG
Plasmid#221661PurposeFor Agrobacterium-mediated transformation. Plastid(thylakoid membrane)-localized mCherry-UnaG.DepositorInsertmCherry-UnaG flanked with the full-length coding sequence for Arabidopsis BESTROPHIN-LIKE PROTEIN2
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIP
Plasmid#79009PurposeFor expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTW170b2
Plasmid#136929PurposeDirected evolution of SpCas9DepositorInsertgVI
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW195b63
Plasmid#136931PurposeDirected evolution of SpCas11DepositorInsertgIII(N)-npuN
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRTagsExpressionBacterialMutationsee manuscriptPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW194b63
Plasmid#136930PurposeDirected evolution of SpCas10DepositorInsertnpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT57
Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT59
Plasmid#223431PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_ATP1A1_G7
Plasmid#173204PurposeExpresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.DepositorInsertATP1A1 G7 sgRNA + SpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only