165,214 results

1. pQCXIP-GFP1-10

   Plasmid

   #68715

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   GFP1-10
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-Lifeact_Halo_T2A_EGFP

   Plasmid

   #135445

   Purpose

   Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   Lifact-HaloTag7 and EGFP
   

   Tags

   Lifact

   Expression

   Mammalian

   Promoter

   CMV/TetO

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EZ-Tet-pLKO-Puro

   Plasmid

   #85966

   Purpose

   Tet/Dox inducible shRNA lentivirus with puromycin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Promoter

   H1 / TetO

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-MMLVgag-dCas9-ZIM3

   Plasmid

   #240527

   Purpose

   Expresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3

   Depositor

   James Nuñez

   Article

   Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

   Insert

   MMLVgag-dCas9-ZIM3
   

   Tags

   FLAG, HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

   Plasmid

   #159905

   Purpose

   Mutagenesis of Slc32a1

   Depositor

   Larry Zweifel

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pER-mScarlet3_N1

   Plasmid

   #189772

   Purpose

   Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   ER-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 SARS-CoV-2 N

   Plasmid

   #158079

   Purpose

   Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 nucleocapsid (N )
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-miniTurbo

   Plasmid

   #124647

   Purpose

   Expresses Flag-miniTurbo fusion for proximity labeling

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Type

   Empty backbone

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-M-IRES-E

   Plasmid

   #177938

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-tag

    Plasmid

    #55180

    Purpose

    FLAG-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    FLAG
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon-optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SNCA-A53T-mRuby3

    Plasmid

    #215379

    Purpose

    Expression of the A53T mutant of alpha-synuclein with a C-terminal mRuby3 tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Tags

    mRuby3 tag

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-pcDNA3.1 Puro-CAG-Positron2

    Plasmid

    #239079

    Purpose

    Mammalian expression of positive-going voltage sensor

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2
    

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    CAG

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid

    #203652

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire-IRES-H2B-mCherry

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. alpha7nACHR_Nacho_GCAMP7s

    Plasmid

    #182940

    Purpose

    alpha7 nACHR expression in mammalian cell lines with calcium reporter

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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