We narrowed to 6,336 results for: WUS;

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RPS15A sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192227

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2AV

Plasmid

#193354

Purpose

Retroviral vector expressing mouse Sox2A61V point mutant

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2AV (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Sox2A61V is a highly cooperative point mutant of …

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2AV

Plasmid

#193352

Purpose

Retroviral vector expressing human SOX2A61V point mutant

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2AV (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

5'LTR

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pVPSBA01-SARS-CoV2-nsp1 mutant4

Plasmid

#192174

Purpose

express SARS2-NSP1-mut4

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVPSBA01-SARS-CoV2-nsp1-truncated

Plasmid

#192172

Purpose

express SARS2-NSP1-trunc

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-mADAR2-short

Plasmid

#175588

Purpose

mammalian expression of mouse short Adar2, blasticidine selection

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Short Mouse Adar2

Tags

Flag

Expression

Mammalian

Promoter

cmv

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

Plasmid

#177767

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

R9A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

Plasmid

#177768

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

S95A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

Plasmid

#177769

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K116A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

Plasmid

#177770

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

T139A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K370A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

Plasmid

#177772

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

S374A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K592A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn-Lrrc23-FLAG

Plasmid

#179921

Purpose

Vector backbone. pClgn-promoter driven expression.

Depositor

Masahito Ikawa

Article

Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Lrrc23 (Lrrc23 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Stop codon removed

Promoter

Calmegin

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn-Lrrc23-1D4

Plasmid

#179922

Purpose

Vector backbone. pClgn-promoter driven expression.

Depositor

Masahito Ikawa

Article

Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Lrrc23 (Lrrc23 Mouse)

Tags

1D4

Expression

Mammalian

Mutation

Stop codon removed

Promoter

Calmegin

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p23-NES-RanCas13b-msfGFP-NES-Flag

Plasmid

#165073

Purpose

overexpression of RanCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RanCas13b

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp7 (SARS-CoV-2)

Plasmid

#169178

Purpose

Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7 (ORF1ab Synthetic, SARS-CoV-2)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265C

Plasmid

#164715

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible haid-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E1

Plasmid

#164718

Purpose

Gateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a-cbe_v01 entry clone

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E3

Plasmid

#164720

Purpose

Gateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

eA3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible ea3a-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA targeting human SLC12A5 gene stop codon

Plasmid

#158577

Purpose

For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

Depositor

Rudolf Jaenisch

Article

Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

Insert

sgRNA targeting human SLC12A5 gene stop codon

Use

CRISPR

Tags

Cas9 and GFP

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_ZFP64_Delta_TAD

Plasmid

#118698

Purpose

Express ZFP64 without transcription activation domain(TAD)

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64_Delta_TAD (ZFP64 Human)

Use

Lentiviral

Tags

3XFLAG was fused to the N terminal of ZFP64 cDNA

Mutation

Delete amino acid from 453 to 681

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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DLG3_PDZ_2

Plasmid

#103947

Purpose

Protein expression and purification of NE-DLG PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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IL16_PDZ_3

Plasmid

#103929

Purpose

Protein expression and purification of IL16 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

IL16 (IL16 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CYTIP_PDZ_1

Plasmid

#103930

Purpose

Protein expression and purification of PSCDBP PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

CYTIP (CYTIP Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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