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hEGFR-mEGFP

Plasmid

#182878

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

Depositor

Salvatore Chiantia

Article

Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

Insert

hEGFR-mEGFP (EGFR Human)

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET23 P53DBD

Plasmid

#174503

Purpose

express P53 DNA binding domain (94-312) in E.coli without tag

Depositor

Stewart Loh

Article

Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

Insert

P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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3XFLAG_ALDOA in pCW57_tTA_Blast

Plasmid

#154210

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCD86-EGFP

Plasmid

#133858

Purpose

Mammalian expression of CD86 C-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pH3R-mCherry-N1

Plasmid

#84327

Purpose

Histamine-3 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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CA-0039 CMVTO-hIL10 (Fused Dimer)

Plasmid

#231187

Purpose

Fused human IL-10 dimer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Fused human IL-10 dimer (IL10 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.MARK2-Halo

Plasmid

#224414

Purpose

Mammalian expression of MARK2

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

MARK2 (MARK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pXG290

Plasmid

#141284

Purpose

OE of HRI

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

HRI (EIF2AK1 Human)

Tags

mClover

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-eIF4E K119A

Plasmid

#199440

Purpose

Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

Depositor

Shin-ichi Hoshino

Article

Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

Insert

1977 (EIF4E Human)

Tags

GST-fusion protein

Expression

Bacterial

Mutation

changed lysine 119 to alanine

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

Plasmid

#233549

Purpose

A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9

Plasmid

#188076

Purpose

Recombinant overexpression of human ATG9 in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Pax6

Plasmid

#32932

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Pax6 (isoform 2, transcript variant 5) (Pax6 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
p2attB-FST344-HA-CPL

Plasmid

#131460

Purpose

Encodes human Follistatin protein

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

Follistatin (FST Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Article

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
TNF-SBP-EGFP_puromycin

Plasmid

#65284

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Caveolin-C-10

Plasmid

#54025

Purpose

Localization: Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-Tubulin-C-18

Plasmid

#55915

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
psiCheck-ApoECDS

Plasmid

#41848

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE CDS (APOE Human)

Use

Luciferase

Tags

renilla luciferase

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only