We narrowed to 60,661 results for: cloning
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Plasmid#113083PurposeEnhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.DepositorTypeEmpty backboneUseCRISPRExpressionWormAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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STAGR_gRNAScaffold_hU6
Plasmid#102840PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_hU6promoter
PromoterhU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106ExpressionBacterialAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialPromoterpPH (Polyhedrin promoter)Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAX
Plasmid#53274PurposeContains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorMutationDeleted idi (ORF6) and crtX genesPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB2_AB
Plasmid#98534Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H3
Plasmid#79514PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDSDepositorInsertLXR Alpha (NR1H3 Human)
UseMultisite gateway entry cloneAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3001
Plasmid#91094PurposeModule C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoter35SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_h7SK
Plasmid#102842PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_h7SKpromoter
UseAs pcr templatePromoterh7SKAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR/zeo-MACROD1
Plasmid#172572PurposeFor gateway cloning of N-terminal tagged MACROD1 constructs.DepositorInsertMACROD1 (MACROD1 Human)
UseGateway cloningAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR/zeo-MACROD2
Plasmid#172573PurposeFor gateway cloning of N-terminal tagged MACROD2 constructs.DepositorInsertMACROD2 (MACROD2 Human)
UseGateway cloningAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C WT
Plasmid#123078PurposeGateway entry clone encoding human MAP1LC3C wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3003
Plasmid#91095PurposeModule C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoterCmYLCVAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
IZ501: pMVP/Lenti-DEST
Plasmid#121848PurposepMVP destination vector, empty Lentivirus vector backboneDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NFAT5
Plasmid#79506PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXTK066 [Con-Pro_nptII-Broad_PartType8]
Plasmid#229303PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8DepositorInsertnptII Promoter (Broad)
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialPromoterT7-LacOAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only