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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLex_Cas9

Plasmid

#117987

Purpose

pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.

Depositor

Paul Khavari

Article

Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

Insert

Cas9-P2A-NLS-BASTR

Use

Lentiviral

Tags

P2A linker, nuclear localization signal and blast…

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-WDR5

Plasmid

#59969

Purpose

Bacterial Expression GST-WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78606

Purpose

pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

173CMV, U6

Available Since

Dec. 16, 2017

Availability

Academic Institutions and Nonprofits only
pTXB1-EXO1b D173A

Plasmid

#68269

Purpose

Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

Depositor

Stefano Ferrari

Article

Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

Insert

EXO1 (EXO1 Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

catalytically-dead D173A. Please see depositor co…

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

Plasmid

#192160

Purpose

Dual AAV vector AAV-dCas9

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Insert

Dead Cas9

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-358-eGFP

Plasmid

#110693

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-eGFP (CPSF6 )

Use

Lentiviral

Tags

EGFP

Mutation

Truncation of CPSF6 isoform 2 at amino acid 358

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22 / PP1gamma

Plasmid

#113511

Purpose

Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Inserts

SDS22 (PPP1R7 Human)
PP1gamma (PPP1CC Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT D554N

Plasmid

#154279

Purpose

Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Aspartate 554 changed to Asparagine- bacterial co…

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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KAT6B-Tol2

Plasmid

#113384

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

KAT6B-enhancer (KAT6B Human)

Use

Tol2 reporter

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only
MAC-TMPRSS4

Plasmid

#172422

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 4 (TMPRSS4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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KAT6B-luciferase

Plasmid

#113353

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

KAT6B-enhancer (KAT6B Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His-mEos3.2

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTYF-PRSx8-AstR-p2A-GFP

Plasmid

#159632

Purpose

Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

Depositor

Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

AstR (AstA-R1 Fly)

Use

Lentiviral

Tags

GFP and P2A

Expression

Mammalian

Promoter

PRSx8

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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FH1-PNGT#2-mCherry

Plasmid

#164099

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Björefeldt et al Neurophotonics. 2024 Apr;11(2):021005. d

Insert

mNeonGreen, eKL9h, and Volvox channelrhodopsin-1

Use

AAV

Promoter

hSyn

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-ECT2

Plasmid

#183835

Purpose

Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 homology arms with mNeonGreen-linker (ECT2 Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C

Plasmid

#171247

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys (AR-W741C)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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shFGF2#2

Plasmid

#157668

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

Plasmid

#10880

Depositor

Patrick Aebischer

Article

Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

Insert

H1-shSOD1 (SOD1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
Mutant Human Cortactin-GFP

Plasmid

#71365

Purpose

mammalian expression of mutant human Cortactin-GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

cortactin (CTTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutation present at 72nd nucleotide

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
UbG76V-eGFP-V5His_15aa

Plasmid

#23969

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_15aa (UBB Human)

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pSICO-CPSF6-358-iRFP670

Plasmid

#110694

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Mutation

Truncation of CPSF6 isoform at amino acid 358

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EphB4-Fc

Plasmid

#200986

Purpose

Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB4

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CDK2-3XFlag-pBabe

Plasmid

#73557

Purpose

vector encoding a fusion protein between CDK2 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK2 (CDK2 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only
pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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