We narrowed to 9,769 results for: 158

1. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlphai1-RLuc8

   Plasmid

   #140973

   Purpose

   Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai1-RLuc8 (GNAI1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

   Plasmid

   #216695

   Purpose

   Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
   

   Use

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-2dV-Camui (wt)

   Plasmid

   #220366

   Purpose

   Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
   

   Use

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

   Plasmid

   #224123

   Purpose

   Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-mutant (Q20A_Y23A_Y24F_F35A)
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Q20A_Y23A_Y24F_F35A in La(SSB)1-194

   Promoter

   CMV

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Beta1

   Plasmid

   #140987

   Purpose

   Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GBeta1 (GNB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal human rhinovirus (HRV) 3C …

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458M-53BP1-DN1S

   Plasmid

   #131045

   Purpose

   Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

   Depositor

   Punam Malik

   Article

   Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

   Insert

   SpCas9-53BP1-DN1S (TP53BP1 Human)
   

   Use

   CRISPR

   Tags

   GFP and SpCas9

   Expression

   Mammalian

   Mutation

   Only expressing residues 1231-1644 of complete 53…

   Promoter

   CBH

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Archon1-KGC-EGFP-ER2-WPRE

   Plasmid

   #108423

   Purpose

   Archon1 fluorescent voltage reporter in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GGamma5-GFP2

    Plasmid

    #166777

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma5-GFP2 (GNG5 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. miniCGBE1-SpRY (pBM1571)

    Plasmid

    #170105

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

    Promoter

    CMV

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-mEGFP-YAP-MAML2

    Plasmid

    #217612

    Purpose

    part A of YAP-MAML2 SPARK-OFF

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    FKBP-mEGFP-YAP-MAML2 (YAP1, MAML2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF2091

    Plasmid

    #144382

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF2038

    Plasmid

    #144327

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PCDH-WT-ALKBH5-3xFLAG

    Plasmid

    #203736

    Purpose

    Overexpression of ALKBH5-WT

    Depositor

    Jianjun Chen

    Article

    Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

    Insert

    ALKBH5 (ALKBH5 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSF143-wArchon1-KGC-EGFP-ER2

    Plasmid

    #108429

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    wArchon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-18

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRetroX GFP Mps1 wt

    Plasmid

    #63702

    Purpose

    Inducible expression of GFP-Mps1 fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    Mps1 (TTK Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Circular 200,100 with interspersed loops (GAPDH)

    Plasmid

    #180183

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Circular 200,100 (mIDUA)

    Plasmid

    #170123

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Idua Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Circular 200,100 with 8 bp bulge loops (GAPDH)

    Plasmid

    #170121

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPR v2 puro - CRBN (#1)

    Plasmid

    #166240

    Purpose

    sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF2036

    Plasmid

    #144205

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF2039

    Plasmid

    #144379

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF3029

    Plasmid

    #144505

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATOH1 (ATOH1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid

    #114078

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid

    #140984

    Purpose

    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha15-RLuc8 (GNA15 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tet-pLKO-puro shKRAS

    Plasmid

    #116871

    Purpose

    Tet-inducible suppression of KRAS

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    shKRAS (KRAS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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