Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,223 results for: Cas9

Clear keywords
Showing: 2921 - 2940 of 14223 results

  1. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

    Enlarge

  3. pCMV-dCas9-mD3A

    Plasmid
    #78257
    Purpose
    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Mutation
    E756A
    Promoter
    pCMV
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78257-plasmid-map-sequence-id-150189

    Enlarge

  7. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

    Enlarge

  11. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

    Enlarge

  12. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104910-plasmid-map-sequence-id-206061

    Enlarge

  15. pcDNA3.1-dCas9-CibN

    Plasmid
    #60552
    Purpose
    Expresses dCas9-CibN in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CibN
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60552-plasmid-map-sequence-id-97051

    Enlarge

  16. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Publication
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, Synthetic, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165602-plasmid-map-sequence-id-327024

    Enlarge

  17. P628_SpCas9-RecA

    Plasmid
    #87263
    Purpose
    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency
    Depositor
    Yonglun Luo
    Publication
    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RecA
    Use
    CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    no
    Promoter
    CBh
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87263-plasmid-map-sequence-id-173851

    Enlarge

  18. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

    Enlarge

Showing: 2921 - 2940 of 14223 results