We narrowed to 18,262 results for: Met;

1. pcDNA3.1-GoldenGate-VP64

   Plasmid

   #47389

   Purpose

   Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   FLAG, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTE101

   Plasmid

   #59396

   Purpose

   MethyloBrick cloning vector, no promoter; KmR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLI-sandwich(0807600)

   Plasmid

   #85790

   Purpose

   P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
   

   Use

   P. falciparum genomic integration

   Tags

   2xFKBP, GFP

   Expression

   Mutation

   Promoter

   none

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLifeAct_mScarlet-H_N1

   Plasmid

   #85055

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-SMVP-Cas9N-U6-gRNA P2

   Plasmid

   #80944

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEos4a-C1

   Plasmid

   #54810

   Purpose

   Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mEos4a

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pcDNA-PhoCl-mCherry-myc

    Plasmid

    #87692

    Purpose

    Express PhoCl-mCherry with C-term myc tag in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl-mCherry-myc
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Use

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTriEx-mCherry-LOVSD

    Plasmid

    #81033

    Purpose

    Mammalian expression of the super-dark state of LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOVSD
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    C450A, L514K, G528A, L531E, N538E

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCytERM_mScarlet-H_N1

    Plasmid

    #85067

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH_SBP-mCherry-Ecadherin

    Plasmid

    #65291

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

17. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. UMG-SP-2_pET29b

    Plasmid

    #215434

    Purpose

    Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

    Plasmid

    #81133

    Purpose

    mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    VAV2 DH/PH/C1 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRNA-U6-let7 sponge

    Plasmid

    #35664

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    U6 let-7sponge
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge