We narrowed to 11,272 results for: VARS

1. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

   Plasmid

   #230058

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Tags

   6X HIS, HRV 3C PreScission protease, HaloTag, and…

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

   Plasmid

   #230059

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Tags

   6X HIS, HRV 3C PreScission protease, HaloTag, and…

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ABE8r-V106W

   Plasmid

   #208295

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-V106W-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pAAV_hSynapsin_psychLight2

   Plasmid

   #163909

   Purpose

   To generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

   Has Service

   AAV9

   Insert

   psychLight2
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAB064d

   Plasmid

   #79197

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   Plac62; Ppro1

   Available Since

   Dec. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-flex-ReaChR-citrine

   Plasmid

   #50955

   Purpose

   flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

   Depositor

   Roger Tsien

   Article

   Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

   Insert

   ReaChR-citrine
   

   Use

   AAV

   Tags

   citrine

   Promoter

   human synapsin promoter

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiV2-hU6-evopreQ1

   Plasmid

   #210189

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ABE8r

    Plasmid

    #208294

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTPH412

    Plasmid

    #185724

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-gp41-8N
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    ecTadA(8e) R26G

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-ReaChR-citrine

    Plasmid

    #50956

    Purpose

    ReaChR-citrine in lentival transfer vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    Lentiviral

    Tags

    citrine

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFCcGi

    Plasmid

    #59324

    Purpose

    Inducible expression of GFP and constitutive expression of mCherry

    Depositor

    David Holden, Sophie Helaine

    Article

    Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

    Inserts

    mCherry
    EGFP
    

    Expression

    Bacterial

    Promoter

    Pbad and rpsM

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-Bxb1o_nlsFlag

    Plasmid

    #119901

    Purpose

    Expresses Bxb1 integrase in mammalian cells

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    Mammalian codon optimized Bxb1 integrase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV7 mitoDendra2

    Plasmid

    #157757

    Purpose

    Expression of the fluorescent protein Dendra2 targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    Dendra2
    

    Expression

    Mammalian

    Mutation

    MTS coxVIII +Dendra2

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. S100A6

    Plasmid

    #196217

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses S100A6.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    S100A6 (S100A6 Human)
    

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge