We narrowed to 10,981 results for: Vars;

1. PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11

   Plasmid

   #216394

   Purpose

   dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)

   Depositor

   Evgeny Kvon

   Article

   Hollingsworth et al bioRxiv 2023.12.10.570890

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human beta-globin

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Cas9-NRCH

   Plasmid

   #136926

   Purpose

   Expresses Cas9-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRCH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+)- PC-MjMAT

   Plasmid

   #179372

   Purpose

   PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

   Depositor

   Andrea Rentmeister

   Article

   Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

   Insert

   PC-MjMAT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L147A, I351A

   Promoter

   T7

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTPH405c

   Plasmid

   #185726

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

   Plasmid

   #200637

   Purpose

   Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   Vif
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T27stop

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDAS12125_PEAR-GFP

   Plasmid

   #177178

   Purpose

   PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.cytGAP

   Plasmid

   #78734

   Purpose

   Expression of Ca2+ sensor GAP in the cytosol

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   cytGAP
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P shACO1

   Plasmid

   #169884

   Purpose

   Suppress ACO1

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   shRNA against ACO1
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-P3-evoCjCas9-TadCDd_gRNA

    Plasmid

    #202563

    Purpose

    P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBA328

    Plasmid

    #214748

    Purpose

    Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RBS(22821)-mcherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-UGI-nCas9(H840A)-GSVG

    Plasmid

    #187411

    Purpose

    DddA GSVG variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xFLAG-p62 PB1mut

    Plasmid

    #204577

    Purpose

    Expresses 3xFLAG-p62 PB1mut in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

    Insert

    p62 (Sqstm1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K7A/D69A

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CMV-evoCjCas9-BE4max-BlastR

    Plasmid

    #194061

    Purpose

    Mammalian expression of evoCjCas9 CBE4max

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. xCas9 3.6

    Plasmid

    #108384

    Purpose

    Mammalian expression vector for xCas9 3.6

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiV2-hU6-evopreQ1

    Plasmid

    #210189

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

    Plasmid

    #192651

    Purpose

    3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-10xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2T-CMV-evoCjCas9-BlastR

    Plasmid

    #194059

    Purpose

    Mammalian expression of evoCjCas9 nuclease

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

    Plasmid

    #159853

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(PATC)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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