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pSF3-ultraID

Plasmid

#172878

Purpose

expresses ultraID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-ATF4 mScarlet NLS

Plasmid

#115969

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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RCIscript-GoldyTALEN

Plasmid

#38142

Purpose

destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

Depositor

Daniel Carlson, Stephen Ekker

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Mrna transcription vector

Tags

AcV5 and FokI homodimer

Mutation

Truncate at N and C terminous

Promoter

T3

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-H2A

Plasmid

#36207

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

H2A (H2AC16 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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MIGR1

Plasmid

#27490

Depositor

Warren Pear

Article

Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM-3z/601

Plasmid

#26656

Depositor

Jonathan Widom

Article

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Insert

Expression

Bacterial

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Skp2

Plasmid

#19947

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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