We narrowed to 3,040 results for: multiplex

1. AAV-KrasG13R/sgKras/Cre

   Plasmid

   #99858

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFD5

   Plasmid

   #73914

   Purpose

   multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   dU6-3:tRNA:gRNA:tRNA:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nTALE+CD_for TUB_8N_1397NC

   Plasmid

   #193383

   Purpose

   nTALECD (1397NC) expression vector

   Depositor

   Shin-ichi Arimura

   Article

   Hosoda et al bioRxiv 2023.02.27.530312

   Insert

   For multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

   Plasmid

   #198511

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-SIO-PdCO-mScarlet-WPRE

   Plasmid

   #198509

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV_hSyn-PdCO-EGFP-WPRE

   Plasmid

   #198513

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV_hSyn-PdCO-mScarlet-WPRE

   Plasmid

   #198510

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

   Plasmid

   #198515

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

   Plasmid

   #198508

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIα minimal promotor (0.4kb)

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

    Plasmid

    #198514

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRL-MND-hCard11 delta e4-T2A-GFP

    Plasmid

    #163771

    Purpose

    Expression of CARD domain variant with a deletion of exon 4

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 4

    Promoter

    MND

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRRL-MND-hCard11 delta e3-4-T2A-GFP

    Plasmid

    #163772

    Purpose

    Expression of CARD domain variant with a deletion of exons 3 and 4.

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 3-4

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRRL-MND-hCard11 delta e3-T2A-GFP

    Plasmid

    #163770

    Purpose

    Expression of CARD domain variant with a deletion of exon 3

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 3

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pECNUS_MP02

    Plasmid

    #194351

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid

    #202199

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid

    #202198

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pECNUS_MP01

    Plasmid

    #194350

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9, FAST-Red
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge