We narrowed to 15,994 results for: LEA

Showing: 2941 - 2960 of 15994 results

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pBAD-LacI

Plasmid

#46394

Purpose

Overproduction of LacI

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI

Tags

6xHIS tag

Expression

Bacterial

Mutation

silent T to C mutation at position 19

Promoter

arabinose promoter

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBAC-lacZ

Plasmid

#13422

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II WT

Plasmid

#35175

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pNuc-TrxRFP1

Plasmid

#98998

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and nuclear localization sequence

Tags

His-tag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

Plasmid

#62346

Purpose

Expresses rtTA2sM2 and tTSkid in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

rtTA2sM2-IRES-tTSkid

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EFS

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Rap1b

Plasmid

#118319

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b (RAP1B Bovine)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n

Plasmid

#47929

Purpose

expression of an optimized Cas9 for genome-editing in zebrafish

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

nls-zcas9-nls

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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KBB464

Plasmid

#185097

Purpose

HA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminus

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

Pom152DGlyc-GFP

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8ChR2 (H134R)eYFP

Plasmid

#89539

Purpose

Expresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Holloway et al Eur J Neurosci. 2015 Sep;42(6):2271-82.

Insert

ChR2 (H134R)

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRVdG-N-P-M-nucBFP-EGFP-L

Plasmid

#52485

Purpose

Expresses nuclear mTagBFP and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

nuclear mTagBFP
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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AP1

Plasmid

#179678

Purpose

Accessory plasmids

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGP4GY-cccdB

Plasmid

#83878

Purpose

Destination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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