We narrowed to 12,214 results for: LEA
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Plasmid#179678PurposeAccessory plasmidsDepositorInsert[SD8] gIII
ExpressionBacterialAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6GRNA
Plasmid#68370PurposegRNA scaffold with hU6 promoterDepositorTypeEmpty backboneExpressionMammalianPromoterhU6Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTETSIX
Plasmid#74104PurposemCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other AlphaproteobacteriaDepositorInserttetA(C)
UseConjugative bacterial vectorMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…Available sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Membrane mNeonGreen
Plasmid#198057PurposePlasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLCK membrane mNeonGreen (LCK Human, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmNeonGreenMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-mScarlet
Plasmid#191098PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro
Plasmid#116876Purposestable TCR expression in human T cellsDepositorUseLentiviralExpressionMammalianPromoterEF1-alphaAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR45Q
Plasmid#92248PurposeHTT donor DNA with 45Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorMutationBarcode of wobble bases to prevent TALEN cleaving…Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR S72T variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_LuxSit_i_mTagBFP2
Plasmid#194300PurposeMammalian expression of the de novo luciferase, LuxSit-iDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuxSit-i
TagsmTagBFP2ExpressionMammalianAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeExpressionBacterialAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPGK-empty
Plasmid#35094DepositorPublicationInsertdeletion of zinc fingers and nuclease
TagsHAExpressionMammalianMutationuse as a transfection balance for pPGK-ZFN plasmi…PromoterPGKAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCNPpo6
Plasmid#46086DepositorInsertPpoI
TagsNLSExpressionMammalianPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCNCre3
Plasmid#46088DepositorInsertCreI
TagsNLSExpressionMammalianPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046747
Plasmid#149249PurposeBacterial expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS
Plasmid#134653Purposelysosome-targeted Sac1 catalytic domain C389SDepositorInsertSac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationC389S and contains only amino acids 77-520 (Pleas…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-Rap1b V12
Plasmid#118323PurposeExpression of bovine Rap1bDepositorAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VCVV
Plasmid#27789DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVenus-Cerulean-Venus-Venus
UseStoichiometry standardExpressionMammalianAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAMBgfp
Plasmid#206130PurposeThis binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.DepositorInserthygromycin resistance gene and the Promoter of ToxA+SGFP
UseBinary vector for expression of sgfp in filamento…Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only