We narrowed to 12,111 results for: LEA
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Plasmid#149249PurposeBacterial expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS
Plasmid#134653Purposelysosome-targeted Sac1 catalytic domain C389SDepositorInsertSac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationC389S and contains only amino acids 77-520 (Pleas…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)
Plasmid#205278PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)DepositorInsertSpuFz1 omegaRNA
ExpressionMammalianAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-N-Strep2
Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX2
Plasmid#112699PurposeExpression of NFIX2 protein and GFPDepositorAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…ExpressionBacterialAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCer-Mcm5
Plasmid#211391Purposemammalian expression of human Mcm5 N-terminally fused to mCeruleanDepositorAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CalfluxCTN
Plasmid#190068PurposeExpresses BRET Ca2+ sensor CalfluxCTN in mammalian cellsDepositorInsertCalfluxCTN
ExpressionMammalianPromoterCAG promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2T-CMV-AIDmax-BlastR
Plasmid#152994PurposeC-to-T base editorDepositorInsertAID-nSpCas9-UGI-UGI
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
NKP38 GFP (1009)
Plasmid#62021Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY019 (pcDNA3.1-hPmCpf1)
Plasmid#69991PurposeExpresses humanized PmCpf1DepositorInserthPmCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY015 (pcDNA3.1-hLiCpf1)
Plasmid#69987PurposeExpresses humanized LiCpf1DepositorInserthLiCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY009 (pcDNA3.1-hSsCpf1)
Plasmid#69981PurposeExpresses humanized SsCpf1DepositorInserthSsCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXcX-PRS-EGFP
Plasmid#25865DepositorInsertPRS driven enhanced green fluorescent protein
ExpressionMammalianAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only