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pTW334e3

Plasmid

#246588

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Nov. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411a

Plasmid

#246582

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN20, CPN60A, AT3G04550 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PD2529 human alpha5 ecto

Plasmid

#221394

Purpose

Mammalian expression of human integrin alpha5 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 ectodomain (ITGA5 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HR…

Expression

Mammalian

Mutation

codon optimized for human mature residues _5 F1 t…

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* full-length

Plasmid

#221402

Purpose

Mammalian expression of human integrin beta1 Q280* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 K418* full-length

Plasmid

#221403

Purpose

Mammalian expression of human integrin beta1 K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* full-length

Plasmid

#221404

Purpose

Mammalian expression of human integrin beta1 N391* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* ectodomain

Plasmid

#221399

Purpose

Mammalian expression of human integrin beta1 Q280* K418* ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N562* full-length

Plasmid

#221405

Purpose

Mammalian expression of human integrin beta1 N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* ectodomain

Plasmid

#221400

Purpose

Mammalian expression of human integrin beta1 N391* N562* ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-ires-mCherry

Plasmid

#209073

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K142R

Plasmid

#213423

Purpose

Expresses human SKP1 K142R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K163R

Plasmid

#213424

Purpose

Expresses human SKP1 K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K163R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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