We narrowed to 7,519 results for: Lif
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Plasmid#187882PurposeGal4/UAS expression of dSarm(isoE,K450R)::FLAGDepositorInsertdSarm isoform E (Sarm )
UseTagsFLAGExpressionMutationK450RPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E)::flag
Plasmid#187871PurposeGal4/UAS expression of dSarm(isoE)::FLAGDepositorInsertdSarm isoform E (Sarm Fly)
UseTagsFLAGExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-QF2 HDR plasmid
Plasmid#141099PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-62-SFFV-T2A-PuroR-polyA-R11b
Plasmid#179893PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-64-SFFV-T2A-BlaR-polyA-R11b
Plasmid#179895PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iDianiSnFR_PM
Plasmid#177751PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iDianiSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iDianiSnFR_ER
Plasmid#177750PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iDianiSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCyt_BrEt_SnFR_PM
Plasmid#177747PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCyt_BrEt_SnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
_pCMV(MinDis)-iCyt_F_SnFR_PM
Plasmid#177745PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsert_pCMV(MinDis)-iCyt_F_SnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCytSnFR_PM
Plasmid#177743PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCytSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCyt_BrEt_SnFR_ER
Plasmid#177746PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCyt_BrEt_SnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
_pCMV(MinDis)-iCyt_F_SnFR_ER
Plasmid#177744PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsert_pCMV(MinDis)-iCyt_F_SnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCytSnFR_ER
Plasmid#177742PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCytSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA6
Plasmid#171004PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A, R538A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/Y
Plasmid#166973PurposeExpresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AS/Y
Plasmid#166974PurposeExpresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)
Plasmid#91806PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationResidues A1529-G1534 replaced with a PreScission …PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
Plasmid#91807PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationPreScission Protease site (LEVLFQGP) inserted bet…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
Plasmid#91818PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutationsDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged lysine 1355 and lysine 1435 to alanines; …PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only