We narrowed to 8,455 results for: Ott

1. p2CT-His-MBP-Lba_Cas13a_WT

   Plasmid

   #91867

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbaCas13a (LbaC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-mScarlet

   Plasmid

   #140875

   Purpose

   Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   mScarlet
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFS461

   Plasmid

   #89064

   Purpose

   leu1 integration plasmid with adh1pr:Z3EV

   Depositor

   Nick Rhind

   Article

   Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

   Insert

   Z3EV artificial transcription factor
   

   Use

   Leu1 integrating vector

   Tags

   Expression

   Mutation

   Promoter

   adh1

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBSV2G_PflaB-msfGFPBb

   Plasmid

   #118231

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Casp1

   Plasmid

   #98849

   Purpose

   Expresses murine Casp1 in mammalian cells

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Casp1 (Casp1 Mouse)
   

   Use

   Tags

   No fusion proteins or tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACAGW-ChR2-Venus-AAV

   Plasmid

   #20071

   Purpose

   AAV-mediated expression of ChR2-Venus for neuronal excitation

   Depositor

   Karel Svoboda

   Article

   Leopoldo et al Nature. 2009 Jan 18.

   Has Service

   AAV1 and AAV9

   Insert

   Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)
   

   Use

   AAV

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-EGFP

   Plasmid

   #140877

   Purpose

   126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EGFP
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 S6K2 WT

   Plasmid

   #17729

   Depositor

   John Blenis

   Article

   Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

   Insert

   S6K2 (RPS6KB2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pME-Lifeact

   Plasmid

   #109545

   Purpose

   Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actin binding motif Lifeact
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLKO-cell-mEm

    Plasmid

    #185595

    Purpose

    Lentiviral construct for expression of mEmerald

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    cell-mEm
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPP444

    Plasmid

    #158802

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ Dkk1-flag

    Plasmid

    #16690

    Depositor

    Xi He

    Article

    Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

    Insert

    Dkk1 (DKK1 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. LZRS-IresGFP

    Plasmid

    #21961

    Depositor

    Lynda Chin

    Article

    Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

    Insert

    Ires GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    GACAGATGACTATGCAGAGAT

    Promoter

    Available since

    Sept. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

15. VinculinTS-A50I

    Plasmid

    #111827

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPP076

    Plasmid

    #158794

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL059

    Plasmid

    #177210

    Purpose

    Bicistronic vector with GFP reporter

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-4xnrUAS

    Plasmid

    #87120

    Purpose

    Multisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elements

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    4xnrUAS (non repetitive)
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBSV2_Psyn-sgRNA500

    Plasmid

    #149613

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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