We narrowed to 6,396 results for: rel

1. MARIO/ pcDNA3

   Plasmid

   #124838

   Purpose

   mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

   Depositor

   Takeharu Nagai

   Article

   Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

   Insert

   MARIO
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41a iGluu

   Plasmid

   #119834

   Purpose

   Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluu
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S72T

   Promoter

   T7

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-GST-4

   Plasmid

   #228986

   Purpose

   For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-4
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-GST-3

   Plasmid

   #228985

   Purpose

   For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-3
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR V5-RNF25 dRING_IRES-mCherry

   Plasmid

   #198387

   Purpose

   RNF25 (?RING) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RNF25 (RNF25 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   RING deletion (135-201)

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDonor-tBFP-NLS-Neo

   Plasmid

   #80766

   Purpose

   Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   TagBFP2-3×NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER13 miR-200c-141

   Plasmid

   #81020

   Purpose

   Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

   Depositor

   Jeffrey Rosen, Thomas Westbrook

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   miR-200c (MIR200C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5155

   Plasmid

   #175391

   Purpose

   Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLR5-CAG-NLS-SNAP

   Plasmid

   #174879

   Purpose

   PiggyBac transposon vector for expression of NLS-SNAP

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   NLS-SNAP
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAGGS-MCS-NLS-3XFLAG-V5

    Plasmid

    #196383

    Purpose

    Empty backbone vector to insert cDNA for strong expression in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3xFLAG and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SENP2 (catalytic domain)

    Plasmid

    #16357

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP2 catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 365 to 590 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-hSOX10

    Plasmid

    #24749

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    wildtype sequence

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCAGGS-N-TEV-Twin-Strep

    Plasmid

    #202526

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLR5-CAG-TetR_W43F-3xmNG

    Plasmid

    #174880

    Purpose

    PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    TetR(W43F)-3xmNeonGreen
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCMV-Tag4A-MITF-M (wt)

    Plasmid

    #31151

    Depositor

    Yardena Samuels

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    MITF-M (MITF Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa.Halo-myosin Va tail 1006-1828

    Plasmid

    #162715

    Purpose

    Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Va (Myo5a Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1005 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AviTag-SpyTag003-MBP

    Plasmid

    #184227

    Purpose

    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    AviTag-SpyTag003-MBP
    

    Use

    Tags

    AviTag, His6, SpyTag003, and Thrombin cleavage si…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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