We narrowed to 6,952 results for: CAR

Showing: 2961 - 2980 of 6952 results

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pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Publication

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Rac1

Plasmid

#55692

Purpose

Expression of mouse Rac1

Depositor

Julian Downward

Publication

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

Rac1 (Rac1 Mouse)

Expression

Bacterial

Promoter

lac

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Publication

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Publication

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47

Plasmid

#127585

Purpose

Expresses mgU2-47 scaRNA from a mU6 promoter

Depositor

David Rueda

Publication

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA

Expression

Mammalian

Promoter

modified U6

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-Sec31-yoHalo

Plasmid

#115423

Purpose

Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec31-yoHalo

Tags

yoHaloTag

Expression

Yeast

Promoter

Endogenous

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Tbx20

Plasmid

#72697

Purpose

Doxycyline regulated expression of Tbx20 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx20 (Tbx20 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLz37_CB_operon_CsoS2_N1-N4

Plasmid

#140856

Purpose

Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Expression

Bacterial

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Publication

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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RhoG

Plasmid

#55694

Purpose

expression of mouse RhoG

Depositor

Julian Downward

Publication

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoG (Rhog Mouse)

Expression

Bacterial

Promoter

lac

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-human LIC1 C-terminal half

Plasmid

#74599

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

Depositor

Ron Vale

Publication

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Mutation

amino acids 389-523 of LIC1

Promoter

T7

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-N-Myc_535-1102 USP7

Plasmid

#131246

Purpose

Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 535-1102. This truncat…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PASTE payload acceptor with attP site pDY2087

Plasmid

#219859

Purpose

Bxb1 payload vector with 5' attP

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

EGFP

Use

CRISPR

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLX302-MKP7-V5 puro

Plasmid

#87771

Purpose

Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP7 (DUSP16 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-4T-1-Skp2 WT

Plasmid

#81117

Purpose

bacterial expression plasmid of Skp2

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only
2xMARS-nGFP

Plasmid

#205235

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

Depositor

Jeremy Baskin

Publication

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-156

Plasmid

#118538

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-ATG5-N-14

Plasmid

#54996

Purpose

Localization: Membrane Receptor, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ATG5 (ATG5 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
Myh6-Cas9

Plasmid

#109037

Purpose

Cardiac-specific overexpression of Cas9

Depositor

Eva van Rooij

Publication

Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

Insert

Cas9

Tags

3XFlag

Expression

Mammalian

Promoter

alpha MHC

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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