We narrowed to 8,372 results for: FIE
-
Plasmid#85590PurposeIntegrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template forDepositorInsertsgRNA targeting firefly luciferase encoding gene
UseCRISPRTagsExpressionBacterialMutationPromoterP3Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-140)-2xFv-Venus
Plasmid#106076Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-140)-2xFv-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationamino acids 1-140PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRM1
Plasmid#157865Purposeexpress His tagged hnRNPA2 RRM1DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PVRL2-bio-His
Plasmid#51824PurposeExpresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPVRL2 (NECTIN2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-hCMV1-hKLF2-IV
Plasmid#60433Purposetet-inducible human KLF4DepositorInsertKLF2 (KLF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y419F
Plasmid#140316PurposeExpresses HA-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationY419FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
ELF3_pet28a
Plasmid#131658PurposeBacterial expression of ETS gene ELF3DepositorInsertELF3 (ELF3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP6 (catalytic domain)
Plasmid#16359DepositorInserthuman SENP6 catalytic domain (SENP6 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 628 to 1112 catalytic domain of human …PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iChR2++
Plasmid#98172Purposeartificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChR2++ gene
UseTagsmCherryExpressionMammalianMutationA59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-GFPL10
Plasmid#98704PurposeCre-dependently express GFPL10 fusion using Introvert SwitchDepositorInsertGFPL10
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationL10: 2609 C->T to remove EcoRI sitePromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai-IRES-GFP
Plasmid#72893PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-hKLF4
Plasmid#60435PurposeOver-expression of human KLF4DepositorInsertKLF4 (KLF4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-EEA1
Plasmid#109560PurposeMultisite gateway vector for 5' tagging with early endosome associated 1DepositorInsertearly endosome associated 1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
P119 C-Check
Plasmid#66817PurposeDual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cellsDepositorInserttruncated Enhanced Green Fluorescent Protein and asRED
UseTagsExpressionMammalianMutationPromoterPGK and CMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-DYRK1B
Plasmid#101771PurposeMammalian expression of DYRK1B fusion proteinDepositorInsertDYRK1B / MIRK (DYRK1B Human)
UseTagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant allelePromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_D290V
Plasmid#139110PurposeExpress FL hnRNPA2 D290V with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationD290VPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
EHF_pet28a
Plasmid#131660PurposeBacterial expression of ETS gene EHFDepositorInsertEHF (EHF Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CDC42
Plasmid#109531PurposeMuscle specific zebrafish expression of mKate2 fused to CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only