We narrowed to 9,006 results for: Pol;

1. Lasso 1

   Plasmid

   #69935

   Purpose

   The first of the two RNA lassos required for the RNA chain construction

   Depositor

   Alfonso Jaramillo

   Article

   Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

   Insert

   Lasso 1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   TAATACGACTCACTATAGGG

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lasso 2

   Plasmid

   #69936

   Purpose

   The second of the two RNA lassos required for the RNA chain construction.

   Depositor

   Alfonso Jaramillo

   Article

   Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

   Insert

   Lasso 2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   TAATACGACTCACTATAGGG

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCyc-Bem1

   Plasmid

   #66573

   Purpose

   Express an additional copy of Bem1, integrated at TRP locus

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   Bem1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F99I*(see below)

   Promoter

   pCyc1

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-KpDcr1deltaC(E224Q, GiVL-2)

   Plasmid

   #32035

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1(15-355)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   E224Q and N195-M215 replaced by P697-D714 of GiDi…

   Promoter

   T7

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T4 Lysozyme L31d-3=L31d WT*

   Plasmid

   #18256

   Depositor

   Brian Matthews

   Article

   Sagermann et al Protein Sci. 2006 May . 15(5):1085-92.

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   insertion of Gly Ile Gly His Leu Leu after I27 WT*

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

6. C150

   Plasmid

   #174350

   Purpose

   Template for making mRNA for overexpressing human eIF4G (C-FLAG) in frog oocytes

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   eIF4G1
   

   Use

   In vitro transcription template

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-BOS-T7-CA150

   Plasmid

   #21924

   Depositor

   Mariano Garcia-Blanco

   Article

   Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

   Insert

   CA150 (TCERG1 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

8. LBJJ383

   Plasmid

   #117517

   Purpose

   PCR template for sgRNA classic with polyT as terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTHSSd_8

   Plasmid

   #59948

   Purpose

   PT7 GFP reporter

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PT7

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. GFP-UBF

    Plasmid

    #17656

    Depositor

    Tom Misteli

    Article

    Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

    Insert

    UBF (UBTF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD33-Gm

    Plasmid

    #65098

    Purpose

    bacterial expression vector with Gentamycin selection, based on pBAD33

    Depositor

    Juan Tomas

    Article

    Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCACTUS

    Plasmid

    #167559

    Purpose

    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

    Type

    Empty backbone

    Use

    Suicide cloning vector, temperature sensitive.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4134347

    Plasmid

    #152890

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAB151

    Plasmid

    #101666

    Purpose

    Opto-T7RNAP*(563-F1)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563-F1)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGFH-14c

    Plasmid

    #39541

    Depositor

    Katharina Strub

    Article

    Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

    Insert

    SRP14 (SRP14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP

    Plasmid

    #18810

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    mGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

18. RPO41

    Plasmid

    #205072

    Purpose

    RPO41 protein expression

    Depositor

    Smita Patel

    Article

    Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

    Insert

    RPO41 (RPO41 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N Terminal 100 amino acids

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACYC-Taq

    Plasmid

    #102793

    Purpose

    Expresses Taq DNA polymerase under WT T7 promoter

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Taq DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac1-melHis10TEV

    Plasmid

    #159428

    Purpose

    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Use

    Tags

    melittin signal sequence (removed in vivo by sign…

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge