We narrowed to 32,062 results for: eng

1. pDisplay-GACh2.0

   Plasmid

   #106073

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 2.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

   Plasmid

   #164510

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.

   Depositor

   Loren Looger

   Article

   Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

   Has Service

   AAV9

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Mutation

   Synthetic Construct

   Promoter

   Synapsin

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. iCAP-BI-28-KanR

   Plasmid

   #203532

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI28 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-syn-Flpo

   Plasmid

   #174378

   Purpose

   also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Has Service

   AAV Retrograde

   Insert

   FLPo recombinase
   

   Use

   AAV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-SC4

   Plasmid

   #236254

   Purpose

   AAV-SC4 capsid which targets peripheral nerves in mice

   Depositor

   Casey Maguire

   Article

   Abou Haidar et al Gene Ther. 2025 Jun 10. doi: 10.1038/s41

   Inserts

   rep/cap AAV9 VP1 with GATPSTQ peptide insert
   rep
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

   Plasmid

   #170823

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA2
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-YE1-BE3-FNLS-CMV-mCherry

   Plasmid

   #154005

   Purpose

   Express YE1-BE3-FNLS

   Depositor

   Hui Yang

   Article

   Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

   Insert

   YE1-BE3-FNLS
   

   Use

   CRISPR

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_rDA-mut

   Plasmid

   #140558

   Purpose

   Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-SUMO-mGSDMD

    Plasmid

    #111560

    Purpose

    Express mouse GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    His, SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac1-Pyl-TAG

    Plasmid

    #228784

    Purpose

    Pyl aaRS/tRNA system for mammalian TAG suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP1101

    Plasmid

    #65773

    Purpose

    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, and T1337R mutations in C…

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ166-SpRY

    Plasmid

    #161520

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9
    

    Use

    CRISPR; Gateway compatible zsprycas9 entry clone

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GACh2.0

    Plasmid

    #121921

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor GACh2.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. iCAP-BI-48-KanR

    Plasmid

    #203533

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI48 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. iCAP-BI-62-KanR

    Plasmid

    #203535

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI62 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro

    Plasmid

    #209645

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100N-T26I&T77I
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-mCardinal-P2A-B19L Flp-in vector

    Plasmid

    #178519

    Purpose

    For making TRE-CB mouse line in Tigre locus

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mCardinal-P2A-B19L
    

    Expression

    Mammalian

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUT8-Dox

    Plasmid

    #124631

    Purpose

    Doxycycline-inducible FUT8 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    

    Expression

    Mammalian

    Promoter

    TRE-tight and hEF1a

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ262B-ABE8e

    Plasmid

    #161524

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA8e-zSpRYCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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