We narrowed to 474 results for: TF

1. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

   Plasmid

   #218508

   Purpose

   integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mNeongreen
   

   Use

   Tags

   modified ssrA degradation tag

   Expression

   Bacterial

   Mutation

   deletion of MCS containing Plac_lacZa, introducti…

   Promoter

   P23

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-DsRed_IRES_MAPT:EGFP

   Plasmid

   #92196

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-MAPT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TPC2-mCherry

   Plasmid

   #135183

   Purpose

   Expresses TPC2 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUCmini-iCAP-PHP.N

   Plasmid

   #127851

   Purpose

   non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.N VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TPC1-mCherry

   Plasmid

   #135182

   Purpose

   Expresses TPC1 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiCRISPR v2-sgAMPK alpha1 clone1

   Plasmid

   #162119

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTFG005_Spike_Inducible_3xFlag

   Plasmid

   #191578

   Purpose

   Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   SARS-CoV-2 Spike Protein (S Synthetic)
   

   Use

   Lentiviral

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG26

   Plasmid

   #226743

   Purpose

   Expresses a reporter for a mitochondrial intermembrane space protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MICU1 (targeting sequence) (MICU1 Human)
   

   Use

   Lentiviral

   Tags

   GFP1-10

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUCmini-iCAP-PHP.V2

    Plasmid

    #127848

    Purpose

    non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUCmini-iCAP-PHP.B4

    Plasmid

    #127849

    Purpose

    non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.B4 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N1 VAMP2 R125TAG

    Plasmid

    #69876

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2-GFP (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amber stop codon in the linker between VAMP2 and …

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGMC00013

    Plasmid

    #172525

    Purpose

    sgRNA against mouse Mr1

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Mr1 (Mr1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGMC00014

    Plasmid

    #172526

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Non-targeting guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pN1 VAMP2 R125TAG

    Plasmid

    #69877

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Amber and Ochre stop codons in the former linker …

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

17. LentiCRISPR v2-sgAMPK alpha1 clone3

    Plasmid

    #162121

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid

    #201539

    Purpose

    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)

    Depositor

    Joshua Leonard

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Inserts

    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG491

    Plasmid

    #226747

    Purpose

    CRISPRi guide RNA against MTCH2 (dual guide)

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MTCH2 (MTCH2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG392

    Plasmid

    #226749

    Purpose

    CRISPRi guide RNA against ATP13A1

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    ATP13A1 (ATP13A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge