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Plasmid#111818PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2ADepositorUseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-DIO-CAG-hM4Di-P2A-dTomato
Plasmid#202037PurposeAAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomatoDepositorInserthM4Di
UseAAVTagsdTomatoExpressionMutationPromoterCAGAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
Plasmid#51087PurposeForebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterCaMKIIa 1.3 kbAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-tdTomato-polyA
Plasmid#174027PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-tdTomato
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHY23-phage-ubc-nls-ha-stdMCP-tdTomato
Plasmid#164044PurposeExpression of stdMCP-tdTomato in mammalian cellsDepositorInsertstdMCP-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-dTomato-2xrGBD
Plasmid#222638PurposeRho reporterDepositorInsertmiRFP680-NES-p2A-dTomato-2xrGBD
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato
Plasmid#176704Purposeneural activity dependent release of sbGLuc from presynaptic vesiclesDepositorInsertsGaussia luciferase, slow burn
dTomato
UseAAVTagsP2A and hPOMC1-26ExpressionMutationPromoterhSynAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-tdTomato-flag-BicD2 594-FKBP
Plasmid#64205PurposeBicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBPDepositorInsertBicaudal D protein (Bicd2 Mouse)
UseTagsFKBP, flag, and tdTomatoExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagExpressionMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-ReaChR-P2A-dTomato
Plasmid#114368Purposeexpresses a Cre-dependent red light-shifted channelrhodopsin variantDepositorInsertReaChR-P2A-dTomato
UseAAVTagsdTomatoExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
Plasmid#18810DepositorInsertmGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP
Plasmid#18811DepositorInsertChx10 (164bp crit)+SV40p-tdTomato-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2
Plasmid#153538PurposeAAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterEF1α1.1Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP
Plasmid#185699PurposeRetroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoterDepositorInsertsTdtomato,
Synaptophysin-EGFP
UseCre/Lox and RetroviralTagsEGFP and T2AExpressionMammalianMutationPromotermDlxAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro
Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorInsertAZGP1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsT2A and dTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA
Plasmid#37089PurposeExpresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro
Plasmid#110831PurposeExpresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.DepositorInsertAzgp1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsdTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE
Plasmid#113853PurposeSuperfolder GFP and tdTomato linked by T2A (V linker)DepositorInsertsfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7
Plasmid#231356PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-CsChrimson-tdTomato HDR plasmid
Plasmid#141100PurposePlasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
Plasmid#182346PurposePacI-flanked CAG-LSL expression cassette with a FNF for positive selectionDepositorInserttdTomato
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB6-Gfap-T2A-tdTomato-FNF-DTA
Plasmid#140271PurposeB6J Gfap-T2A-tdTomato allele targeting vector, low copy numberDepositorInsertGfap (Gfap Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tdTomatoExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-sfGFP-tdTomato T2A Q XE
Plasmid#113852PurposeSuperfolder GFP and tdTomato linked by T2A (Q linker)DepositorInsertsfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE
Plasmid#113843PurposeHistone H2B-superfolder GFP and tdTomato linked by T2A (V linker)DepositorInsertH2B-sfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE
Plasmid#113842PurposeHistone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)DepositorInsertH2B-sfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
Plasmid#84468PurposepAAV-CRAM-tdTomatoDepositorInserttdTomato
UseAAVTagsExpressionMutationT230I (please see depositor comments below)PromoterTREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus
Plasmid#61629PurposeR2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoterDepositorInsertsmDII
ntdTomato
DII
n3xVenus
UseTagsExpressionPlantMutationPromoterRPS5AAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
Plasmid#51083PurposeCre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV RetrogradeInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas ServiceAAV1InserttdTomato-T2A-SypEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#84446PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.DepositorHas ServiceAAV1, AAV5, and AAV8InsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#153536PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterSynAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV(cmv)-NEO-FSF-tdTomato Cre reporter
Plasmid#160551Purposelabeling hypoxic cells, Cre reporterDepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#195488PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro
Plasmid#89991PurposeDonor template for generation of SOX2-ntdTomato reporter cell linesDepositorInsertSOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
Plasmid#51082PurposeCre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV1InsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMEH303 NLS dTomato 6x high affinity MS2 loops
Plasmid#168215PurposeMammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)
Plasmid#166610PurposeEncodes Cre-inactivated ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVTagsExpressionMutationH134RPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH280 NLS dTomato 3x high affinity MS2 loops
Plasmid#168211PurposeMammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only