Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 4,251 results for: Gca

Showing: 701 - 750 of 4251 results

  1. pLCHKO_SFRS7_exon_deletion_4

    Plasmid
    #155072
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155072-plasmid-map-sequence-id-308512

    Enlarge

  2. pLCHKOv3-CD46 Ex3_2-Lb

    Plasmid
    #209028
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209028-plasmid-map-sequence-id-414792

    Enlarge

  3. pLCHKOv3-CD46 Ex3_2-As

    Plasmid
    #209032
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209032-plasmid-map-sequence-id-413524

    Enlarge

  7. pMVP-SEC24C-G220C

    Plasmid
    #231560
    Purpose
    pMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (G220C) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    G220C, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231560-plasmid-map-sequence-id-460046

    Enlarge

  8. pMVP-SEC24C

    Plasmid
    #231559
    Purpose
    pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    silent PAM mutations (G67 (GGG>GGA) and A115 (…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231559-plasmid-map-sequence-id-459740

    Enlarge

  9. pMVP-SEC24C-S107F

    Plasmid
    #231561
    Purpose
    pMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (S107F) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    S107F, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231561-plasmid-map-sequence-id-459737

    Enlarge

  10. pAAV CAG Barcode2

    Plasmid
    #229063
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229063-plasmid-map-sequence-id-455086

    Enlarge

  11. pAAV Syn Barcode19

    Plasmid
    #226196
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode19
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226196-plasmid-map-sequence-id-447886

    Enlarge

  12. pAAV Syn Barcode22

    Plasmid
    #226193
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode22
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226193-plasmid-map-sequence-id-447883

    Enlarge

  13. pCfb13201

    Plasmid
    #219876
    Purpose
    The base plasmid of TUNEYALI forTF11
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF11 (YALI1_E11341g) and homologous arm matching TF11
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219876-plasmid-map-sequence-id-435396

    Enlarge

  14. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Insert
    AAVS1-CAGCas9IRESneo
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166026-plasmid-map-sequence-id-328056

    Enlarge

  15. pICSL4723_ Rac4

    Plasmid
    #126884
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126884-plasmid-map-sequence-id-249713

    Enlarge

  16. pENTRY4_ Rac4

    Plasmid
    #126896
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126896-plasmid-map-sequence-id-250171

    Enlarge

  17. bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

    Plasmid
    #177236
    Purpose
    Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgSnrk
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177236-plasmid-map-sequence-id-352699

    Enlarge

  18. pHnS KI;Actb Donor;3xV5 KO;Dlg4

    Plasmid
    #240292
    Purpose
    KI:Actb Donor:3xV5 KO:Dlg4
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Actb
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240292-plasmid-map-sequence-id-478297

    Enlarge

  19. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Article
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182746-plasmid-map-sequence-id-358079

    Enlarge

  20. pHK352.3

    Plasmid
    #235473
    Purpose
    Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235473-plasmid-map-sequence-id-469104

    Enlarge

  25. pHK302.6

    Plasmid
    #235471
    Purpose
    Message phagemid carrying sgRNA6 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235471-plasmid-map-sequence-id-468578

    Enlarge

  26. pHK052.3

    Plasmid
    #235472
    Purpose
    Message phagemid carrying sgRNA3 (prom. J23119, backbone RSF1030)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235472-plasmid-map-sequence-id-469106

    Enlarge

  27. pHK002.1

    Plasmid
    #235460
    Purpose
    Message phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235460-plasmid-map-sequence-id-468584

    Enlarge

  28. pHK002.2

    Plasmid
    #235461
    Purpose
    Message phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235461-plasmid-map-sequence-id-468611

    Enlarge

  29. pHK002.3

    Plasmid
    #235462
    Purpose
    Message phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235462-plasmid-map-sequence-id-468582

    Enlarge

  30. pHK002.4

    Plasmid
    #235463
    Purpose
    Message phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235463-plasmid-map-sequence-id-468586

    Enlarge

  31. pHK002.5

    Plasmid
    #235464
    Purpose
    Message phagemid carrying sgRNA5 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA5
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235464-plasmid-map-sequence-id-468612

    Enlarge

  32. pHK002.6

    Plasmid
    #235465
    Purpose
    Message phagemid carrying sgRNA6 (prom. J23119, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235465-plasmid-map-sequence-id-468609

    Enlarge

  33. pHK302.1

    Plasmid
    #235466
    Purpose
    Message phagemid carrying sgRNA1 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235466-plasmid-map-sequence-id-468602

    Enlarge

  34. pHK302.2

    Plasmid
    #235467
    Purpose
    Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235467-plasmid-map-sequence-id-468575

    Enlarge

  35. pHK302.3

    Plasmid
    #235468
    Purpose
    Message phagemid carrying sgRNA3 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235468-plasmid-map-sequence-id-468585

    Enlarge

  36. pHK302.4

    Plasmid
    #235469
    Purpose
    Message phagemid carrying sgRNA4 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235469-plasmid-map-sequence-id-468580

    Enlarge

  37. pHK302.5

    Plasmid
    #235470
    Purpose
    Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA5
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235470-plasmid-map-sequence-id-468571

    Enlarge

  46. pUC57-CmYLCV

    Plasmid
    #205245
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CmYLCV
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205245-plasmid-map-sequence-id-408849

    Enlarge

Showing: 701 - 750 of 4251 results