We narrowed to 4,332 results for: U6 gRNA

1. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

   Plasmid

   #209783

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   miR122 ts
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

   Plasmid

   #83306

   Purpose

   Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

   Depositor

   Tohru Kimura

   Article

   Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

   Insert

   sgRNA and dCas9 from pX330
   

   Use

   Lentiviral

   Tags

   FLAG and PA tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and CBh for dCas9

   Available since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

   Plasmid

   #109317

   Purpose

   Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

   Plasmid

   #234724

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

   Plasmid

   #208111

   Purpose

   Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

   Insert

   SpG C, U6, scaffold
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

   Plasmid

   #194726

   Purpose

   CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1(D908A)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

   Plasmid

   #109315

   Purpose

   Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   Mttp gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

    Plasmid

    #180273

    Purpose

    Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

    Plasmid

    #226916

    Purpose

    Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, inteinC, nSp Cas9C
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-U6

    Plasmid

    #120358

    Purpose

    piggyBac transposon vector for sgRNA expression.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-petRNA

    Plasmid

    #181802

    Purpose

    petRNA cloning plasmid

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    petRNA-Kan
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-miR.FF4

    Plasmid

    #235256

    Purpose

    Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FF4 hairpin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    CB

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209062

    Purpose

    Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    non-targeting sgRNA (C0111)
    

    Use

    Gateway vector to be used for lr reaction

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJET1.2-U6

    Plasmid

    #173157

    Purpose

    Single guide RNA cassette under U6 promoter

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Insert

    sgRNA cassette under U6 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR

    Plasmid

    #154001

    Purpose

    Vector expressed CasRx and sgRNA_Ptbp1 for AAV package

    Depositor

    Hui Yang

    Article

    Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

    Insert

    CasRx, Ptbp1 sgRNAs
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    GFAP, U6

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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